CST3 ELISA Kit

Produktnummer ABIN2042433

Dieses Human CST3 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human CST3 zu quantifizieren.

Kurzübersicht für CST3 ELISA Kit (ABIN2042433)

Target: CST3 (Cystatin C (CST3))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 2-20000 pg/mL

Applikation: ELISA

Produktdetails CST3 ELISA Kit

Untere Nachweisgrenze: 2 pg/mL

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile: 96-well plate pre-coated with anti-Cystatin C, Human
Lyophilized recombinant Cystatin C, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Sandwich ELISA quantitative detection kit for Human Cystatin C in cell culture supernates, serum, plasma ( eparin, EDTA), saliva, urine and human milk.Cystatin C or cystatin 3 (formerly gamma trace, post-gamma-globulin or neuroendocrine basic polypeptide), a protein encoded by the CST3 gene, was originally described as a constituent of normal cerebrospinal fluid (CSF) and of urine from patients with renal failure.1 Cystatin 3 has a low molecular weight (approximately 13.3 kilodaltons), and it is removed from the bloodstream by glomerular filtration in the kidneys. In humans, all cells with a nucleus (cell core containing the DNA) produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and bodily fluids. Cystatin C, which belongs to the type II cystatin gene family, is a potent inhibitor of lysosomal proteinases2 (enzymes from a special subunit of the cell that break down proteins) and probably one of the most important extracellular inhibitors of cysteine proteases3 (it prevents the breakdown of proteins outside the cell by a specific type of protein degrading enzymes). Moreover, cystatin C is involved in network reorganization in the epileptic dentate gyrus.4

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu CST3

Target: CST3 (Cystatin C (CST3))

Andere Bezeichnung: Cystatin C

Gen-ID: 1471

UniProt: P01034