Chitinase 3-Like 1/YKL-40 ELISA Kit

Produktnummer ABIN2042405

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human Chitinase 3-Like 1/YKL-40.

Kurzübersicht für Chitinase 3-Like 1/YKL-40 ELISA Kit (ABIN2042405)

Target: Chitinase 3-Like 1/YKL-40

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5-4000 pg/mL

Applikation: ELISA

Produktdetails

Untere Nachweisgrenze: 62.5 pg/mL

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile: 96-well plate pre-coated with anti-Chitinase 3-like 1 /YKL-40, Human
Lyophilized recombinant Chitinase 3-like 1 /YKL-40, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Sandwich ELISA quantitative detection kit for Human Chitinase 3-like 1 /YKL-40 in cell culture supernates, serum, plasma (heparin, EDTA) and urine.Chitinase-3-like protein 1, also called CHI3L1 or YKL-40 is a secreted glycoprotein that is approximately 40kD in size that in humans is encoded by the CHI3L1 gene. This gene is mapped to 1q32.1. Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Antigendetails

Target: Chitinase 3-Like 1/YKL-40

Gen-ID: 1116

UniProt: P36222