NOTCH2NL ELISA Kit

Produktnummer ABIN2041984

Human NOTCH2NL ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human NOTCH2NL.

Kurzübersicht für NOTCH2NL ELISA Kit (ABIN2041984)

Target: NOTCH2NL (Notch 2 N-terminal Like (NOTCH2NL))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails NOTCH2NL ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.117 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Notch2 N-Terminal Like Protein (NOTCH2NL) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Notch2 N-Terminal Like Protein (NOTCH2NL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Notch2 N-Terminal Like Protein (NOTCH2NL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Notch2 N-Terminal Like Protein (NOTCH2NL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Notch2 N-Terminal Like Protein (NOTCH2NL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu NOTCH2NL

Target: NOTCH2NL (Notch 2 N-terminal Like (NOTCH2NL))

Andere Bezeichnung: Notch2 N-Terminal Like Protein (NOTCH2NL)