CRP ELISA Kit

Produktnummer ABIN1889453

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human CRP. Es wurde in 5 Publikationen zitiert.

Kurzübersicht für CRP ELISA Kit (ABIN1889453)

Target: CRP (C-Reactive Protein (CRP))

Bindungsspezifität: AA 17-224

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 312 pg/mL - 20000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Produktdetails CRP ELISA Kit

Untere Nachweisgrenze: 312 pg/mL

Verwendungszweck: Sandwich Human CRP/C Reactive Protein ELISA Kit to quantitate Human CRP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NS0
Immunogen sequence: F17-P224

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Testpräzision: Intra-Assay Precision (CV%): 6.1%, 4.2%, 7.9%
Inter-Assay Precision (CV%): 6.8%, 4.9%, 9.8%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Haltbarkeit: 12 months

Referenzen für CRP ELISA Kit (ABIN1889453)

Koh, Park: "Responses of inflammatory cytokines following moderate intensity walking exercise in overweight or obese individuals." in: Journal of exercise rehabilitation, Vol. 13, Issue 4, pp. 472-476, (2017) (PubMed).

Liu, Piao, Guo, Wang, Sun, Gao, Zheng, Fang: "A New Chinese Medicine Intestine Formula Greatly Improves the Effect of Aminosalicylate on Ulcerative Colitis." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2017, pp. 7323129, (2017) (PubMed).

Asgary, Keshvari, Sahebkar, Hashemi, Rafieian-Kopaei: "Clinical investigation of the acute effects of pomegranate juice on blood pressure and endothelial function in hypertensive individuals." in: ARYA atherosclerosis, Vol. 9, Issue 6, pp. 326-31, (2014) (PubMed).

Shi, Song, Zhang, Li, Li: "Correlation between the microinflammatory state and left ventricular structural and functional changes in maintenance haemodialysis patients." in: Experimental and therapeutic medicine, Vol. 6, Issue 2, pp. 532-536, (2013) (PubMed).

Iori, Vinci, Murphy, Marescotti, Avogaro, Ahluwalia: "Glucose and fatty acid metabolism in a 3 tissue in-vitro model challenged with normo- and hyperglycaemia." in: PLoS ONE, Vol. 7, Issue 4, pp. e34704, (2012) (PubMed).

Details zu CRP

Target: CRP (C-Reactive Protein (CRP))

Andere Bezeichnung: C-Reactive Protein

Hintergrund:

Background: C Reactive Protein (CRP) is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is composed of 5 identical, 21,500-molecular weight subunits. CRP mediates activities associated with preimmune nonspecific host resistance. CRP shows the strongest association with cardiovascular events. It is detectable on the surface of about 4 % of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver.

Gene Full Name: C-reactive protein

Gen-ID: 1401

UniProt: P02741

Pathways: Carbohydrate Homeostasis