CRP ELISA Kit

Produktnummer ABIN1889453

Produktdetails CRP ELISA Kit

Target: CRP (C-Reactive Protein (CRP))

Bindungsspezifität: AA 17-224

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56-100 ng/mL

Untere Nachweisgrenze: 1.56 ng/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CRP

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: F17-P224

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: F17-P224

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Belongs to the pentaxin family.

Tissue Specificity: Found in plasma.

Plattentyp: Pre-coated

Protokoll: human CRP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CRP has been precoated onto 96-well plates. Standards(NSO, F17-P224) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CRP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CRP amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 1.25 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL human CRP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CRP standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(ng/ml): 16.1, Standard deviation: 0.080, CV(%): 5
• Sample 2: n=16, Mean(ng/ml): 35, Standard deviation: 1.925, CV(%): 5.5
• Sample 3: n=16, Mean(ng/ml): 60, Standard deviation: 4.32, CV(%): 7.2,
• Sample 1: n=24, Mean(ng/ml): 13.6, Standard deviation: 1.088, CV(%): 6.8
• Sample 2: n=24, Mean(ng/ml): 32.8, Standard deviation: 2.099, CV(%): 6.4
• Sample 3: n=24, Mean(ng/ml): 61, Standard deviation: 4.453, CV(%): 7.3

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für CRP ELISA Kit (ABIN1889453)

Koh, Park: "Responses of inflammatory cytokines following moderate intensity walking exercise in overweight or obese individuals." in: Journal of exercise rehabilitation, Vol. 13, Issue 4, pp. 472-476, (2017) (PubMed).

Liu, Piao, Guo, Wang, Sun, Gao, Zheng, Fang: "A New Chinese Medicine Intestine Formula Greatly Improves the Effect of Aminosalicylate on Ulcerative Colitis." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2017, pp. 7323129, (2017) (PubMed).

Asgary, Keshvari, Sahebkar, Hashemi, Rafieian-Kopaei: "Clinical investigation of the acute effects of pomegranate juice on blood pressure and endothelial function in hypertensive individuals." in: ARYA atherosclerosis, Vol. 9, Issue 6, pp. 326-31, (2014) (PubMed).

Shi, Song, Zhang, Li, Li: "Correlation between the microinflammatory state and left ventricular structural and functional changes in maintenance haemodialysis patients." in: Experimental and therapeutic medicine, Vol. 6, Issue 2, pp. 532-536, (2013) (PubMed).

Iori, Vinci, Murphy, Marescotti, Avogaro, Ahluwalia: "Glucose and fatty acid metabolism in a 3 tissue in-vitro model challenged with normo- and hyperglycaemia." in: PLoS ONE, Vol. 7, Issue 4, pp. e34704, (2012) (PubMed).

Details zu CRP

Target: CRP (C-Reactive Protein (CRP))

Andere Bezeichnung: C-Reactive Protein (CRP Produkte)

Hintergrund:

Protein Function: Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.

Background: C Reactive Protein (CRP) is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is composed of 5 identical, 21,500-molecular weight subunits. CRP mediates activities associated with preimmune nonspecific host resistance. CRP shows the strongest association with cardiovascular events. It is detectable on the surface of about 4 % of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver.

Synonyms: C-reactive protein,C-reactive protein(1-205),CRP,PTX1,

Full Gene Name: C-reactive protein

Cellular Localisation: Secreted.

Gen-ID: 1401

UniProt: P02741

Pathways: Carbohydrate Homeostasis