Sialoadhesin/CD169 ELISA Kit

Produktnummer ABIN1889444

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human Sialoadhesin/CD169.

Kurzübersicht für Sialoadhesin/CD169 ELISA Kit (ABIN1889444)

Target: Sialoadhesin/CD169 (SIGLEC1) (Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1))

Bindungsspezifität: AA 20-1641

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156-10.000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Produktdetails Sialoadhesin/CD169 ELISA Kit

Untere Nachweisgrenze: 156 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CD169/SIGLEC-1

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: S20-Q1641

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: S20-Q1641

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.

Tissue Specificity: Expressed by macrophages in various tissues. High levels are found in spleen, lymph node, perivascular macrophages in brain and lower levels in bone marrow, liver Kupffer cells and lamina propria of colon and lung. Also expressed by inflammatory macrophages in rheumatoid arthritis.

Plattentyp: Pre-coated

Protokoll: human CD169 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD169 has been precoated onto 96-well plates. Standards(NSO, S20-Q1641) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD169 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD169 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human CD169 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CD169 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(ng/ml): 1.55, Standard deviation: 0.065, CV(%): 4.2
• Sample 2: n=16, Mean(ng/ml): 5.23, Standard deviation: 0.199, CV(%): 3.8
• Sample 3: n=16, Mean(ng/ml): 6.77, Standard deviation: 0.305, CV(%): 4.5,
• Sample 1: n=24, Mean(ng/ml): 2.05, Standard deviation: 0.098, CV(%): 4.8
• Sample 2: n=24, Mean(ng/ml): 5.33, Standard deviation: 0.245, CV(%): 4.6
• Sample 3: n=24, Mean(ng/ml): 7.86, Standard deviation: 0.417, CV(%): 5.3

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu Sialoadhesin/CD169

Target: Sialoadhesin/CD169 (SIGLEC1) (Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1))

Andere Bezeichnung: SIGLEC1

Hintergrund:

Protein Function: Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid (By similarity). Binds to SPN/CD43 on T-cells (By similarity). May play a role in hemopoiesis. .

Background: SIGLEC-1, also known as Sialoadhesin or CD169, is a cell adhesion molecule found on the surface of certain cells of the immune system called macrophages. This gene is mapped to 20p13. It belongs to the immunoglobulin superfamily(IgSF). Since sialoadhesin binds sialic acids with its N-terminal IgV-domain, it is also a member of the SIGLEC family. The localization and expression of SIGLEC-1 in humans has altered coincident with the evolutionary loss of Neu5Gc. SIGLEC-1-positive macrophages have a dual physiologic function. They act as innate 'flypaper' by preventing the systemic spread of lymph-borne pathogens and as critical gatekeepers at the lymph-tissue interface that facilitate the recognition of particulate antigens by B cells and initiate humoral immune responses.

Synonyms: Sialoadhesin,Sialic acid-binding Ig-like lectin 1,Siglec-1,CD169,SIGLEC1,SN,

Full Gene Name: Sialoadhesin

Cellular Localisation: Isoform 1: Cell membrane, Single-pass type I membrane protein.

Gen-ID: 6614

UniProt: Q9BZZ2