DMP1 ELISA Kit

Produktnummer ABIN1889432

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Maus DMP1.

Kurzübersicht für DMP1 ELISA Kit (ABIN1889432)

Target: DMP1 (DMTF1) (Cyclin D Binding Myb-Like Transcription Factor 1 (DMTF1))

Bindungsspezifität: AA 17-503

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156-10.000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Tissue Homogenate

Produktdetails DMP1 ELISA Kit

Untere Nachweisgrenze: 156 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse DMP-1

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: L17-Y503

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: L17-Y503

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Tissue Specificity: Expressed in tooth particularly in odontoblast, ameloblast and cementoblast. Also expressed in bone particularly in osteoblast.

Plattentyp: Pre-coated

Protokoll: mouse DMP-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for DMP-1 has been precoated onto 96-well plates. Standards(NSO, L17-Y503) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for DMP-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse DMP-1 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse DMP-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum, plasma(heparin, EDTA) or tissue homogenates to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse DMP-1 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 1680, Standard deviation: 70.56, CV(%): 4.2
• Sample 2: n=16, Mean(pg/ml): 4025, Standard deviation: 246, CV(%): 6.1
• Sample 3: n=16, Mean(pg/ml): 6562, Standard deviation: 374, CV(%): 5.7,
• Sample 1: n=24, Mean(pg/ml): 1553, Standard deviation: 89.96, CV(%): 5.8
• Sample 2: n=24, Mean(pg/ml): 3977, Standard deviation: 290, CV(%): 7.3
• Sample 3: n=24, Mean(pg/ml): 6454, Standard deviation: 419.51, CV(%): 6.5

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu DMP1

Target: DMP1 (DMTF1) (Cyclin D Binding Myb-Like Transcription Factor 1 (DMTF1))

Andere Bezeichnung: DMP1

Hintergrund:

Protein Function: May have a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts, unphosphorylated form acts as a transcriptional component for activation of osteoblast-specific genes like osteocalcin. During the osteoblast to osteocyte transition phase it is phosphorylated and exported into the extracellular matrix, where it regulates nucleation of hydroxyapatite (By similarity). .

Background: Dentin matrix acidic phosphorprotein 1 is a protein that in humans is encoded by the DMP1 gene. It belongs to the small integrin-binding ligand N-linked glycoprotein(SIBLING) family of secreted phosphoproteins. This gene is mapped to 4q22.1. This protein, which is critical for proper mineralization of bone and dentin, is present in diverse cells of bone and tooth tissues. In undifferentiated osteoblasts, DMP-1 is primarily a nuclear protein that regulates the expression of osteoblast-specific genes. During osteoblast maturation, the protein becomes phosphorylated and is exported to the extracellular matrix, where it orchestrates mineralized matrix formation. Mutations in the gene are known to cause autosomal recessive hypophosphatemia, a disease that manifests as rickets and osteomalacia. The gene structure is conserved in mammals.

Synonyms: Dentin matrix acidic phosphoprotein 1,DMP-1,Dentin matrix protein 1,AG1,Dmp1,Dmp,

Full Gene Name: Dentin matrix acidic phosphoprotein 1

Cellular Localisation: Nucleus . Cytoplasm . Secreted, extracellular space, extracellular matrix . In proliferating preosteoblasts it is nuclear, during early maturation stage is cytoplasmic and in mature osteoblast localizes in the mineralized matrix. Export from the nucleus of differentiating osteoblast is triggered by the release of calcium from intracellular stores followed by a massive influx of this pool of calcium into the nucleus (By similarity)..

Gen-ID: 13406

UniProt: O55188