CD6 ELISA Kit

Produktnummer ABIN1889429

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Maus CD6.

Kurzübersicht für CD6 ELISA Kit (ABIN1889429)

Target: CD6

Bindungsspezifität: AA 18-396

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5-4000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Produktdetails CD6 ELISA Kit

Untere Nachweisgrenze: 62.5 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CD6

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: L18-G396

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: L18-G396

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Tissue Specificity: Expressed predominantly in thymus, lymph node and spleen.

Plattentyp: Pre-coated

Protokoll: mouse CD6 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD6 has been precoated onto 96-well plates. Standards(NSO, L18-G396) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD6 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD6 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse CD6 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CD6 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 798, Standard deviation: 33.5, CV(%): 4.2
• Sample 2: n=16, Mean(pg/ml): 1880, Standard deviation: 67.68, CV(%): 3.6
• Sample 3: n=16, Mean(pg/ml): 3505, Standard deviation: 189.27, CV(%): 5.4,
• Sample 1: n=24, Mean(pg/ml): 1036, Standard deviation: 60, CV(%): 5.8
• Sample 2: n=24, Mean(pg/ml): 2040, Standard deviation: 95.88, CV(%): 4.7
• Sample 3: n=24, Mean(pg/ml): 3487, Standard deviation: 240.6, CV(%): 6.9

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu CD6

Target: CD6

Andere Bezeichnung: CD6

Hintergrund:

Protein Function: Involved in cell adhesion. Binds to CD166.

Background: CD6(Cluster of Differentiation 6) is a human protein encoded by the CD6 gene. It is mapped to 11q12.2. CD6 is expressed predominantly in peripheral T cells and mature medullary thymocytes. This gene encodes a protein found on the outer membrane of T-lymphocytes as well as some other immune cells. The encoded protein contains three scavenger receptor cysteine-rich (SRCR) domains and a binding site for an activated leukocyte cell adhesion molecule. The gene product is important for continuation of T cell activation. What’s more, this gene may be associated with susceptibility to multiple sclerosis.

Synonyms: T-cell differentiation antigen CD6,CD6,Cd6,

Full Gene Name: T-cell differentiation antigen CD6

Cellular Localisation: Cell membrane, Single-pass type I membrane protein.

Gen-ID: 12511

UniProt: Q61003