IL18BP ELISA Kit

Produktnummer ABIN1889424

Dieses Human IL18BP ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human IL18BP zu quantifizieren.

Kurzübersicht für IL18BP ELISA Kit (ABIN1889424)

Target: IL18BP (Interleukin 18 Binding Protein (IL18BP))

Bindungsspezifität: AA 31-194

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 93.7-6000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine

Produktdetails IL18BP ELISA Kit

Untere Nachweisgrenze: 93.7 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-18BP

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: T31-G194

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: T31-G194

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Tissue Specificity: Strongly expressed in heart, lung, placenta and spleen. .

Plattentyp: Pre-coated

Protokoll: human IL-18BP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-18BP has been precoated onto 96-well plates. Standards(NSO, T31-G194) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-18BP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-18BP amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 6000pg/mL, 3000pg/mL, 1500pg/mL, 750pg/mL, 375pg/mL, 187.5pg/mL, 93.7pg/m human IL-18BP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IL-18BP standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 1289, Standard deviation: 61.9, CV(%): 4.8
• Sample 2: n=16, Mean(pg/ml): 3561, Standard deviation: 203, CV(%): 5.7
• Sample 3: n=16, Mean(pg/ml): 5320, Standard deviation: 228.76, CV(%): 4.3,
• Sample 1: n=24, Mean(pg/ml): 999, Standard deviation: 51.95, CV(%): 5.2
• Sample 2: n=24, Mean(pg/ml): 2895, Standard deviation: 189, CV(%): 6.5
• Sample 3: n=24, Mean(pg/ml): 4672, Standard deviation: 262, CV(%): 5.6

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu IL18BP

Target: IL18BP (Interleukin 18 Binding Protein (IL18BP))

Andere Bezeichnung: IL18BP

Hintergrund:

Protein Function: Isoform A binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response. .

Background: Interleukin-18-binding protein, also known as IL18BPa, is a protein that in humans is encoded by the IL18BP gene. It is mapped to 11q13.4. The protein encoded by this gene is an inhibitor of the proinflammatory cytokine IL18. This protein binds to IL18, prevents the binding of IL18 to its receptor, and thus inhibits IL18-induced IFN-gamma production. This protein is constitutively expressed and secreted in mononuclear cells. The expression of this protein can be enhanced by IFN-gamma. It has been found that IL18BP expression in intestinal tissue is increased in endothelial cells as well as cells of the submucosa and overlying lymphoid aggregates in Crohn disease.

Synonyms: Interleukin-18-binding protein,IL-18BP,Tadekinig-alfa,IL18BP,

Full Gene Name: Interleukin-18-binding protein

Cellular Localisation: Secreted.

Gen-ID: 10068

UniProt: O95998