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IL18BP ELISA Kit

Dieses Human IL18BP ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human IL18BP zu quantifizieren.
Produktnummer ABIN1889424

Kurzübersicht für IL18BP ELISA Kit (ABIN1889424)

Target

Alle IL18BP ELISA Kits anzeigen
IL18BP (Interleukin 18 Binding Protein (IL18BP))

Bindungsspezifität

AA 31-194

Reaktivität

  • 5
  • 3
  • 1
Human

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

93.7-6000 pg/mL

Applikation

ELISA

Proben

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine
  • Untere Nachweisgrenze

    93.7 pg/mL

    Verwendungszweck

    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-18BP

    Marke

    PicoKine™

    Analytische Methode

    Quantitative

    Spezifität

    Expression system for standard: NSO
    Immunogen sequence: T31-G194

    Kreuzreaktivität (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität

    <10pg/mL

    Benötigtes Material

    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

    Immunogen

    Expression system for standard: NSO
    Immunogen sequence: T31-G194
  • Applikationshinweise

    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

    Kommentare

    Sequence similarities: Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

    Tissue Specificity: Strongly expressed in heart, lung, placenta and spleen. .

    Plattentyp

    Pre-coated

    Protokoll

    human IL-18BP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-18BP has been precoated onto 96-well plates. Standards(NSO, T31-G194) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-18BP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-18BP amount of sample captured in plate.

    Testdurchführung

    Aliquot 0.1 mL per well of the 6000pg/mL, 3000pg/mL, 1500pg/mL, 750pg/mL, 375pg/mL, 187.5pg/mL, 93.7pg/m human IL-18BP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IL-18BP standard solution and each sample be measured in duplicate.

    Testpräzision

    • Sample 1: n=16, Mean(pg/ml): 1289, Standard deviation: 61.9, CV(%): 4.8
    • Sample 2: n=16, Mean(pg/ml): 3561, Standard deviation: 203, CV(%): 5.7
    • Sample 3: n=16, Mean(pg/ml): 5320, Standard deviation: 228.76, CV(%): 4.3,
    • Sample 1: n=24, Mean(pg/ml): 999, Standard deviation: 51.95, CV(%): 5.2
    • Sample 2: n=24, Mean(pg/ml): 2895, Standard deviation: 189, CV(%): 6.5
    • Sample 3: n=24, Mean(pg/ml): 4672, Standard deviation: 262, CV(%): 5.6

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Handhabung

    Avoid multiple freeze-thaw cycles.

    Lagerung

    -20 °C,4 °C

    Informationen zur Lagerung

    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

    Haltbarkeit

    12 months
  • Target Alle IL18BP ELISA Kits anzeigen

    IL18BP (Interleukin 18 Binding Protein (IL18BP))

    Andere Bezeichnung

    IL18BP

    Hintergrund

    Protein Function: Isoform A binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response. .

    Background: Interleukin-18-binding protein, also known as IL18BPa, is a protein that in humans is encoded by the IL18BP gene. It is mapped to 11q13.4. The protein encoded by this gene is an inhibitor of the proinflammatory cytokine IL18. This protein binds to IL18, prevents the binding of IL18 to its receptor, and thus inhibits IL18-induced IFN-gamma production. This protein is constitutively expressed and secreted in mononuclear cells. The expression of this protein can be enhanced by IFN-gamma. It has been found that IL18BP expression in intestinal tissue is increased in endothelial cells as well as cells of the submucosa and overlying lymphoid aggregates in Crohn disease.

    Synonyms: Interleukin-18-binding protein,IL-18BP,Tadekinig-alfa,IL18BP,

    Full Gene Name: Interleukin-18-binding protein

    Cellular Localisation: Secreted.

    Gen-ID

    10068

    UniProt

    O95998
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