DLL1 ELISA Kit

Produktnummer ABIN1889418

Dieses Human DLL1 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human DLL1 zu quantifizieren.

Kurzübersicht für DLL1 ELISA Kit (ABIN1889418)

Target: DLL1 (delta-Like 1 (DLL1))

Bindungsspezifität: AA 22-540

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78-5000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine, Milk

Produktdetails DLL1 ELISA Kit

Untere Nachweisgrenze: 78 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human DLL1

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: S22-G540

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: S22-G540

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Contains 1 DSL domain.

Tissue Specificity: Expressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney.

Plattentyp: Pre-coated

Protokoll: human DLL1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for DLL1 has been precoated onto 96-well plates. Standards(NSO, S22-G540) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for DLL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human DLL1 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL, 78pg/mL human DLL1 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), urine or human milk to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human DLL1 standard solution and each sample is measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 713, Standard deviation: 24.9, CV(%): 3.5
• Sample 2: n=16, Mean(pg/ml): 1579, Standard deviation: 64.74, CV(%): 4.1
• Sample 3: n=16, Mean(pg/ml): 3618, Standard deviation: 137.5, CV(%): 3.8,
• Sample 1: n=24, Mean(pg/ml): 674, Standard deviation: 49.8, CV(%): 7.4
• Sample 2: n=24, Mean(pg/ml): 1486, Standard deviation: 101, CV(%): 6.8
• Sample 3: n=24, Mean(pg/ml): 3542, Standard deviation: 269.2, CV(%): 7.6

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu DLL1

Target: DLL1 (delta-Like 1 (DLL1))

Andere Bezeichnung: DLL1

Hintergrund:

Protein Function: Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor. .

Background: DLL1(DELTA-LIKE 1), also known as DL1 or DELTA1, is a protein that in humans is encoded by the DLL1 gene. DLL1 is a human homolog of the Notch Delta ligand and is a member of the delta/serrate/jagged family. It plays a role in mediating cell fate decisions during hematopoiesis, and it is also important in cell-to-cell communication. This gene is mapped to chromosome 6q27, near a locus for type I diabetes. Functional analysis suggested that a soluble fusion protein containing the DSL domain of DLL1 and its adjacent 50 N-terminal amino acids increased the viability of hemopoietic cells but inhibited cell death. It has been found that DLL1 blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.

Synonyms: Delta-like protein 1,Drosophila Delta homolog 1,Delta1,H-Delta-1,DLL1,UNQ146/PRO172,

Full Gene Name: Delta-like protein 1

Cellular Localisation: Membrane, Single-pass type I membrane protein.

Gen-ID: 28514

UniProt: O00548

Pathways: Notch Signalweg, Stem Cell Maintenance