GAL4 ELISA Kit

Produktnummer ABIN1889417

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human GAL4.

Kurzübersicht für GAL4 ELISA Kit (ABIN1889417)

Target: GAL4 (LGALS4) (Galectin 4 (LGALS4))

Bindungsspezifität: AA 2-323

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156-10.000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Produktdetails GAL4 ELISA Kit

Untere Nachweisgrenze: 156 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Galectin-4

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: E.coli
Immunogen sequence: A2-I323

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: A2-I323

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Contains 2 galectin domains.

Plattentyp: Pre-coated

Protokoll: human Galectin-4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Galectin-4 has been precoated onto 96-well plates. Standards(E.coli, A2-I323) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Galectin-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Galectin-4 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Galectin-4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Galectin-4 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(ng/ml): 0.48, Standard deviation: 0.02, CV(%): 4.2
• Sample 2: n=16, Mean(ng/ml): 3.64, Standard deviation: 0.14, CV(%): 3.8
• Sample 3: n=16, Mean(ng/ml): 6.25, Standard deviation: 0.35, CV(%): 5.6,
• Sample 1: n=24, Mean(ng/ml): 0.51, Standard deviation: 0.03, CV(%): 5.9
• Sample 2: n=24, Mean(ng/ml): 4.02, Standard deviation: 0.19, CV(%): 4.7
• Sample 3: n=24, Mean(ng/ml): 6.93, Standard deviation: 0.43, CV(%): 6.2

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu GAL4

Target: GAL4 (LGALS4) (Galectin 4 (LGALS4))

Andere Bezeichnung: LGALS4

Hintergrund:

Protein Function: Galectin that binds lactose and a related range of sugars. May be involved in the assembly of adherens junctions.

Background: Galectin-4, also known as LGALS4 or GAL4, is a protein that in humans is encoded by the LGALS4 gene. It is mapped to 19q13.2. Galectins are a family of animal lectins defined by an affinity for beta-galactoside-containing saccharides and by shared sequence elements. Galectin-4 is expressed as a 38-kD protein in the human colon adenocarcinoma T84 cell line. Galectin-4 is an S-type lectin that is strongly underexpressed in colorectal cancer, and this gene plays a role in cell adhesion. It has been found that decreased Galectin-4 expression occurs early in colorectal carcinogenesis.

Synonyms: Galectin-4,Gal-4,Antigen NY-CO-27,L-36 lactose-binding protein,L36LBP,Lactose-binding lectin 4,LGALS4,

Full Gene Name: Galectin-4

Gen-ID: 3960

UniProt: P56470