Trefoil Factor 2 ELISA Kit

Produktnummer ABIN1889395

Human Trefoil Factor 2 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human Trefoil Factor 2.

Kurzübersicht für Trefoil Factor 2 ELISA Kit (ABIN1889395)

Target: Trefoil Factor 2 (TFF2)

Bindungsspezifität: AA 24-129

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 7.8-500 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Produktdetails Trefoil Factor 2 ELISA Kit

Untere Nachweisgrenze: 7.8 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TFF2

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: E24-Y129

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: E24-Y129

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Contains 2 P-type (trefoil) domains.

Tissue Specificity: Stomach.

Plattentyp: Pre-coated

Protokoll: human TFF2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TFF2 has been precoated onto 96-well plates. Standards(NSO, E24-Y129) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TFF2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TFF2 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL human TFF2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TFF2 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 62, Standard deviation: 2.3, CV(%): 3.7
• Sample 2: n=16, Mean(pg/ml): 126, Standard deviation: 5.3, CV(%): 4.2
• Sample 3: n=16, Mean(pg/ml): 255, Standard deviation: 13.77, CV(%): 5.4,
• Sample 1: n=24, Mean(pg/ml): 58, Standard deviation: 2.67, CV(%): 4.6
• Sample 2: n=24, Mean(pg/ml): 133, Standard deviation: 7.85, CV(%): 5.9
• Sample 3: n=24, Mean(pg/ml): 263, Standard deviation: 17.62, CV(%): 6.7

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu Trefoil Factor 2

Target: Trefoil Factor 2 (TFF2)

Andere Bezeichnung: TFF2

Hintergrund:

Protein Function: Inhibits gastrointestinal motility and gastric acid secretion. Could function as a structural component of gastric mucus, possibly by stabilizing glycoproteins in the mucus gel through interactions with carbohydrate side chains (By similarity). .

Background: Trefoil factor 2, also known as SP or SML1, is a protein that in humans is encoded by the TFF2 gene. This gene belongs to trefoil family, and members of the trefoil family may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. This gene is mapped to 21q22.3. TFF2 gene consists of 4 exons spanning 5.1 kb, with exons 2 and 3 encoding 1 trefoil domain each, and it is the only gene encoding 2 TFF-domains on separate exons. It has been found that the distribution of TFF2 and pS2 suggests involvement in repair-enhancing mechanisms.

Synonyms: Trefoil factor 2,Spasmolysin,Spasmolytic polypeptide,SP,TFF2,SML1,

Full Gene Name: Trefoil factor 2

Cellular Localisation: Secreted.

Gen-ID: 7032

UniProt: Q03403