PLAUR ELISA Kit

Produktnummer ABIN1889380

Maus PLAUR ELISA-Kit Colorimetric-Assay zur Quantifizierung von Maus PLAUR.

Kurzübersicht für PLAUR ELISA Kit (ABIN1889380)

Target: PLAUR (Plasminogen Activator, Urokinase Receptor (PLAUR))

Bindungsspezifität: AA 24-297

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5-4000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Produktdetails PLAUR ELISA Kit

Untere Nachweisgrenze: 62.5 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse uPAR

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: L24-T297

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <5pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: L24-T297

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Tissue Specificity: Expressed in angiogenic endothelial cells (at protein level). .

Plattentyp: Pre-coated

Protokoll: mouse uPAR ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for uPAR has been precoated onto 96-well plates. Standards(NSO, L24-T297) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for uPAR is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse uPAR amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse uPAR standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse uPAR standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 256, Standard deviation: 13.06, CV(%): 5.1
• Sample 2: n=16, Mean(pg/ml): 1862, Standard deviation: 8.38, CV(%): 4.5
• Sample 3: n=16, Mean(pg/ml): 3015, Standard deviation: 114.6, CV(%): 3.8,
• Sample 1: n=24, Mean(pg/ml): 362, Standard deviation: 23.2, CV(%): 6.4
• Sample 2: n=24, Mean(pg/ml): 2165, Standard deviation: 114.7, CV(%): 5.3
• Sample 3: n=24, Mean(pg/ml): 3230, Standard deviation: 190.6, CV(%): 5.9

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu PLAUR

Target: PLAUR (Plasminogen Activator, Urokinase Receptor (PLAUR))

Andere Bezeichnung: PLAUR

Hintergrund:

Protein Function: Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA.

Background: The urokinase-type plasminogen activator receptor(uPAR) is a key molecule in the regulation of cell-surface plasminogen activation and, as such, plays an important role in many normal as well as pathological processes.1 The cDNA for Mo3, an activation antigen expressed by human monocytes and myelomonocytic cell lines after stimulation by a variety of agents. Mo3 expression in vivo is associated predominantly with macrophages in inflammatory sites. It is a highly glycosylated protein of about 50 kD in monocytes where it is anchored to the plasma membrane by glycosyl-phosphatidylinositol linkage. The complete coding sequence of the cDNA has been found to encode 335 amino acids including a predicted signal peptide of 22 residues and a hydrophobic C-terminal portion. Mo3 is identical to the human receptor for the urokinase plasminogen activator.2 UPAR is a useful prognostic marker for biologically aggressive forms of endometrial cancer.3 PLAUR is located at chromosome 19q13.1-q13.2.1

Synonyms: Urokinase plasminogen activator surface receptor,U-PAR,uPAR,CD87,Plaur,

Full Gene Name: Urokinase plasminogen activator surface receptor

Cellular Localisation: Isoform 1: Cell membrane, Lipid-anchor, GPI- anchor.

Gen-ID: 18793

UniProt: P35456

Pathways: Inositol Metabolic Process