APCS ELISA Kit

Produktnummer ABIN1889377

Maus APCS ELISA-Kit Colorimetric-Assay zur Quantifizierung von Maus APCS.

Kurzübersicht für APCS ELISA Kit (ABIN1889377)

Target: APCS (Amyloid P Component, Serum (APCS))

Bindungsspezifität: AA 21-224

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56-100 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Produktdetails APCS ELISA Kit

Untere Nachweisgrenze: 1.56 ng/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse SAP/PTX2

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: Q21-D224

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <50pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: Q21-D224

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Belongs to the pentaxin family.

Plattentyp: Pre-coated

Protokoll: mouse SAP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for SAP has been precoated onto 96-well plates. Standards(NSO, Q21-D224) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SAP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse SAP amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 1.25 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL mouse SAP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse SAP standard solution and each sample is measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(ng/ml): 5.68, Standard deviation: 0.33, CV(%): 5.8
• Sample 2: n=16, Mean(ng/ml): 34, Standard deviation: 1.43, CV(%): 4.2
• Sample 3: n=16, Mean(ng/ml): 61, Standard deviation: 3.05, CV(%): 5,
• Sample 1: n=24, Mean(ng/ml): 5.2, Standard deviation: 0.39, CV(%): 7.5
• Sample 2: n=24, Mean(ng/ml): 39, Standard deviation: 2.11, CV(%): 5.4
• Sample 3: n=24, Mean(ng/ml): 74, Standard deviation: 4.59, CV(%): 6.2

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu APCS

Target: APCS (Amyloid P Component, Serum (APCS))

Andere Bezeichnung: APCS

Hintergrund:

Background: Amyloid P component, serum(SAP), also known as PTX2 or APCS, is the identical serum form of amyloid P component(AP), a 25 kDa pentameric protein first identified as the pentagonal constituent of in vivo pathological deposits called "amyloid". It belongs to the pentraxins family, characterised by calcium dependent ligand binding and distinctive flattened beta-jellyroll structure similar to that of the legume lectins. This gene is mapped to 1q23.2. The binding of the encoded protein to proteins in the pathological amyloid cross-beta fold suggests its possible role as a chaperone. This protein is also thought to control the degradation of chromatin. It has been demonstrated that this protein binds to apoptotic cells at an early stage, which raises the possibility that it is involved in dealing with apoptotic cells in vivo.

Synonyms: Serum amyloid P-component,SAP,Apcs,Ptx2, Sap,

Full Gene Name: Serum amyloid P-component

Cellular Localisation: Secreted.

UniProt: P12246