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MSMB ELISA Kit (Microseminoprotein, beta) ELISA Kit

MSMB Reaktivität: Human AA 21-114 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Produktnummer ABIN1889376
$369.00
Zzgl. Versandkosten $45.00
96 tests
Lieferung in 4 bis 6 Werktagen
  • Target Alle MSMB ELISA Kits anzeigen
    MSMB
    Bindungsspezifität
    AA 21-114
    Reaktivität
    • 6
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    62.5-4000 pg/mL
    Untere Nachweisgrenze
    62.5 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human PSP94
    Marke
    PicoKine™
    Proben
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytische Methode
    Quantitative
    Spezifität
    Expression system for standard: E.coli
    Immunogen sequence: S21-I114
    Kreuzreaktivität (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivität
    <10pg/mL
    Benötigtes Material
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: E.coli
    Immunogen sequence: S21-I114
    Featured
    Zu unserem meistverkauften MSMB ELISA Kit
  • Applikationshinweise
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Kommentare

    Sequence similarities: Belongs to the beta-microseminoprotein family.

    Tissue Specificity: Strongly expressed in prostate, liver, kidney, breast and penis. Also expressed in pancreas, esophagus, stomach, deodenum, colon, trachea, lung, salivary glands and fallopian tube. PSP94 is expressed in lung and breast, whereas PSP57 is found in kidney and bladder. .

    Plattentyp
    Pre-coated
    Protokoll
    human PSP94 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PSP94 has been precoated onto 96-well plates. Standards(E.coli, S21-I114) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PSP94 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human PSP94 amount of sample captured in plate.
    Testdurchführung

    Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human PSP94 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human PSP94 standard solution and each sample be measured in duplicate.

    Testpräzision
    • Sample 1: n=16, Mean(pg/ml): 765, Standard deviation: 36.72, CV(%): 4.8
    • Sample 2: n=16, Mean(pg/ml): 1533, Standard deviation: 78.2, CV(%): 5.1
    • Sample 3: n=16, Mean(pg/ml): 2897, Standard deviation: 182.5, CV(%): 6.3,
    • Sample 1: n=24, Mean(pg/ml): 890, Standard deviation: 56.96, CV(%): 6.4
    • Sample 2: n=24, Mean(pg/ml): 1915, Standard deviation: 137.88, CV(%): 7.2
    • Sample 3: n=24, Mean(pg/ml): 3143, Standard deviation: 238.9, CV(%): 7.6
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Avoid multiple freeze-thaw cycles.
    Lagerung
    -20 °C,4 °C
    Informationen zur Lagerung
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Haltbarkeit
    12 months
  • Target Alle MSMB ELISA Kits anzeigen
    MSMB
    Andere Bezeichnung
    MSMB (MSMB Produkte)
    Synonyme
    HPC13, IGBF, MSP, MSPB, PN44, PRPS, PSP, PSP-94, PSP57, PSP94, MSMB, PIP, beta-MSP, microseminoprotein beta, microseminoprotein, beta, beta-microseminoprotein, microseminoprotein, beta-, MSMB, Msmb
    Hintergrund

    Background: Beta-microseminoprotein, also known as MSMB or PSP94, is a protein that in humans is encoded by the MSMB gene. It is a member of the immunoglobulin binding factor family. This gene is mapped to 10q11.23. It is synthesized by the epithelial cells of the prostate gland and secreted into the seminal plasma. The expression of the encoded protein is found to be decreased in prostate cancer. But transcripts of identical size were found in prostate, respiratory(tracheal, bronchial, and lung) tissues, and the mucosa of the gastric antrum. Thus, the protein may have functions other than those related to male reproduction. This protein has inhibin-like activity. It may have a role as an autocrine paracrine factor in uterine, breast and other female reproductive tissues.

    Synonyms: Beta-microseminoprotein,Immunoglobulin-binding factor,IGBF,PN44,Prostate secreted seminal plasma protein,Prostate secretory protein of 94 amino acids,PSP-94,PSP94,Seminal plasma beta-inhibin,MSMB,PRSP,

    Full Gene Name: Beta-microseminoprotein

    Cellular Localisation: Secreted. Sperm surface.
    Gen-ID
    4477
    UniProt
    P08118
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