SAA1 ELISA Kit

Produktnummer ABIN1889362

Maus SAA1 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Maus SAA1.

Kurzübersicht für SAA1 ELISA Kit (ABIN1889362)

Target: SAA1 (Serum Amyloid A1 (SAA1))

Bindungsspezifität: AA 20-122

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56-100 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine

Produktdetails SAA1 ELISA Kit

Untere Nachweisgrenze: 1.56 ng/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse SAA

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: E.coli
Immunogen sequence: G20-Y122

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <150pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: G20-Y122

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Tissue Specificity: Detected in blood plasma (at protein level). Detected in liver. .

Plattentyp: Pre-coated

Protokoll: mouse SAA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for SAA has been precoated onto 96-well plates. Standards(E.coli, G20-Y122) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SAA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse SAA amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse SAA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse SAA standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(ng/ml): 1.4, Standard deviation: 0.06, CV(%): 4.3
• Sample 2: n=16, Mean(ng/ml): 3.4, Standard deviation: 0.177, CV(%): 5.2
• Sample 3: n=16, Mean(ng/ml): 5.7, Standard deviation: 0.291, CV(%): 5.1,
• Sample 1: n=24, Mean(ng/ml): 1.5, Standard deviation: 0.081, CV(%): 5.4
• Sample 2: n=24, Mean(ng/ml): 3.6, Standard deviation: 0.223, CV(%): 6.2
• Sample 3: n=24, Mean(ng/ml): 6.8, Standard deviation: 0.388, CV(%): 5.7

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu SAA1

Target: SAA1 (Serum Amyloid A1 (SAA1))

Andere Bezeichnung: SAA1

Hintergrund:

Protein Function: Major acute phase protein. .

Background: Serum amyloid A protein(SAA), also known as SAA1, is a protein that in humans is encoded by the SAA1 gene. This gene encodes a member of the serum amyloid A family of apolipoproteins. It is mapped to 11p15.1. SAA is a major acute phase protein that is highly expressed in response to inflammation and tissue injury. This protein also plays an important role in HDL metabolism and cholesterol homeostasis. High levels of this protein are associated with chronic inflammatory diseases including atherosclerosis, rheumatoid arthritis, Alzheimer's disease and Crohn's disease. This protein may also be a potential biomarker for certain tumors.

Synonyms: Serum amyloid A-1 protein,Saa1,

Full Gene Name: Serum amyloid A-1 protein

Cellular Localisation: Secreted.

Gen-ID: 111345

UniProt: P05366

Pathways: Toll-Like Receptors Cascades