TIMP2 ELISA Kit

Produktnummer ABIN1889329

Maus TIMP2 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Maus TIMP2 und wurde in 6+ Publikationen erwähnt.

Kurzübersicht für TIMP2 ELISA Kit (ABIN1889329)

Target: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Bindungsspezifität: AA 27-220

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156-10000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Produktdetails TIMP2 ELISA Kit

Untere Nachweisgrenze: 156 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse TIMP-2

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: C27-P220

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: C27-P220

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Belongs to the protease inhibitor I35 (TIMP) family.

Tissue Specificity: Detected in testis, retina, hippocampus and cerebral cortex. .

Plattentyp: Pre-coated

Protokoll: mouse TIMP-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TIMP-2 has been precoated onto 96-well plates. Standards(NSO, C27-P220) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TIMP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TIMP-2 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse TIMP-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse TIMP-2 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(ng/ml): 1.24, Standard deviation: 0.056, CV(%): 4.5
• Sample 2: n=16, Mean(ng/ml): 3.85, Standard deviation: 0.146, CV(%): 3.8
• Sample 3: n=16, Mean(ng/ml): 6.85, Standard deviation: 0.219, CV(%): 3.2,
• Sample 1: n=24, Mean(ng/ml): 1.32, Standard deviation: 0.088, CV(%): 6.7
• Sample 2: n=24, Mean(ng/ml): 3.96, Standard deviation: 0.245, CV(%): 6.2
• Sample 3: n=24, Mean(ng/ml): 6.88, Standard deviation: 0.516, CV(%): 7.5

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für TIMP2 ELISA Kit (ABIN1889329)

Papakonstantinou, Karakiulakis, Batzios, Savic, Roth, Tamm, Stolz: "Acute exacerbations of COPD are associated with significant activation of matrix metalloproteinase 9 irrespectively of airway obstruction, emphysema and infection." in: Respiratory research, Vol. 16, pp. 78, (2016) (PubMed).

Gishto, Farrell, Kothapalli: "Tuning composition and architecture of biomimetic scaffolds for enhanced matrix synthesis by murine cardiomyocytes." in: Journal of biomedical materials research. Part A, Vol. 103, Issue 2, pp. 693-708, (2015) (PubMed).

Cavdar, Ozbal, Celik, Ergur, Guneli, Ural, Camsari, Guner: "The effects of alpha-lipoic acid on MMP-2 and MMP-9 activities in a rat renal ischemia and re-perfusion model." in: Biotechnic & histochemistry : official publication of the Biological Stain Commission, Vol. 89, Issue 4, pp. 304-14, (2014) (PubMed).

Xu, Ling, Zhu, Fan, Zhang: "The effect of 2,3,4',5-tetrahydroxystilbene-2-0-?-D glucoside on neointima formation in a rat artery balloon injury model and its possible mechanisms." in: European journal of pharmacology, Vol. 698, Issue 1-3, pp. 370-8, (2013) (PubMed).

Kim, Lee, Choi, Yoo, Yang: "Implication of MMP-9 and urokinase plasminogen activator (uPA) in the activation of pro-matrix metalloproteinase (MMP)-13." in: Rheumatology international, Vol. 32, Issue 10, pp. 3069-75, (2012) (PubMed).

Yu, Zhu, Mi, Chen, Pan, Wei: "Anti-angiogenic genistein inhibits VEGF-induced endothelial cell activation by decreasing PTK activity and MAPK activation." in: Medical oncology (Northwood, London, England), Vol. 29, Issue 1, pp. 349-57, (2012) (PubMed).

Details zu TIMP2

Target: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Andere Bezeichnung: TIMP2

Hintergrund:

Protein Function: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.

Background: TIMP-2 gen is encoded by 5 exons spanning 83 kb of genomic DNA. TIMP-2 is 83 kilobase pairs (kb) long with exon-intron splicing sites located in preserved positions among the three members of the TIMP family. The gene for tissue inhibitor of metalloproteinases-2 is localized on human chromosome arm 17q25. TIMP-2 abrogates angiogenic factor-induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition. The standard product used in this kit is recombinant human TIMP-2 with the molecular mass of 22Kda and 194 amino acid.

Synonyms: Metalloproteinase inhibitor 2,Tissue inhibitor of metalloproteinases 2,TIMP-2,Timp2,Timp-2,

Full Gene Name: Metalloproteinase inhibitor 2

Cellular Localisation: Secreted.

Gen-ID: 21858

UniProt: P25785

Pathways: cAMP Metabolic Process