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ICAM-3/CD50 ELISA Kit

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human ICAM-3/CD50. Es ist 1 Publikation verfügbar.
Produktnummer ABIN1889327

Kurzübersicht für ICAM-3/CD50 ELISA Kit (ABIN1889327)

Target

Alle ICAM-3/CD50 (ICAM3) ELISA Kits anzeigen
ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

Bindungsspezifität

AA 30-485

Reaktivität

  • 6
  • 1
  • 1
Human

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

156-10000 pg/mL

Applikation

ELISA

Proben

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
  • Untere Nachweisgrenze

    156 pg/mL

    Verwendungszweck

    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human ICAM-3

    Marke

    PicoKine™

    Analytische Methode

    Quantitative

    Spezifität

    Expression system for standard: NSO
    Immunogen sequence: Q30-H485
    Immunogen sequence: homodimer

    Kreuzreaktivität (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität

    <10pg/mL

    Benötigtes Material

    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

    Immunogen

    Expression system for standard: NSO, Q30-H485
    Immunogen sequence: homodimer
  • Applikationshinweise

    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

    Kommentare

    Sequence similarities: Belongs to the immunoglobulin superfamily. ICAM family.

    Plattentyp

    Pre-coated

    Protokoll

    human ICAM-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ICAM-3 has been precoated onto 96-well plates. Standards(NSO, Q30-H485, homodimer) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ICAM-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ICAM-3 amount of sample captured in plate.

    Testdurchführung

    Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human ICAM-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human ICAM-3 standard solution and each sample be measured in duplicate.

    Testpräzision

    • Sample 1: n=16, Mean(ng/ml): 0.58, Standard deviation: 0.021, CV(%): 3.7
    • Sample 2: n=16, Mean(ng/ml): 2.73, Standard deviation: 0.123, CV(%): 4.5
    • Sample 3: n=16, Mean(ng/ml): 5.45, Standard deviation: 0.294, CV(%): 5.4,
    • Sample 1: n=24, Mean(ng/ml): 0.68, Standard deviation: 0.04, CV(%): 5.9
    • Sample 2: n=24, Mean(ng/ml): 3.16, Standard deviation: 0.215, CV(%): 6.8
    • Sample 3: n=24, Mean(ng/ml): 6.24, Standard deviation: 0.443, CV(%): 7.1

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Handhabung

    Avoid multiple freeze-thaw cycles.

    Lagerung

    -20 °C,4 °C

    Informationen zur Lagerung

    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

    Haltbarkeit

    12 months
  • Huang, Huang, Xiao, Yang, Lin, Diao: "Kallistatin, a novel anti-angiogenesis agent, inhibits angiogenesis via inhibition of the NF-?B signaling pathway." in: Biomedicine & pharmacotherapy = Biome?decine & pharmacothe?rapie, Vol. 68, Issue 4, pp. 455-61, (2014) (PubMed).

  • Target Alle ICAM-3/CD50 (ICAM3) ELISA Kits anzeigen

    ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

    Andere Bezeichnung

    ICAM3

    Hintergrund

    Protein Function: ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2.

    Background: Intercellular adhesion molecule 3(ICAM3) also known as CD50(Cluster of Differentiation 50), is a human gene. The protein encoded by this gene is a member of the intercellular adhesion molecule(ICAM) family. ICAM3 is closely related to ICAM1, consists of 5 immunoglobulin domains, and binds LFA1 through its 2 N-terminal domains. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. ICAM3 has been shown to interact with EZR and Moesin.

    Synonyms: Intercellular adhesion molecule 3,ICAM-3,CDw50,ICAM-R,CD50,ICAM3,

    Full Gene Name: Intercellular adhesion molecule 3

    Cellular Localisation: Membrane, Single-pass type I membrane protein.

    Gen-ID

    3385

    UniProt

    P32942
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