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MST1 ELISA Kit

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human MST1.
Produktnummer ABIN1889321

Kurzübersicht für MST1 ELISA Kit (ABIN1889321)

Target

Alle MST1 ELISA Kits anzeigen
MST1 (Macrophage Stimulating 1 (Hepatocyte Growth Factor-Like) (MST1))

Bindungsspezifität

AA 19-711

Reaktivität

  • 4
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Human

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

156-10000 pg/mL

Applikation

ELISA

Proben

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
  • Untere Nachweisgrenze

    156 pg/mL

    Verwendungszweck

    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human MSP/MST1

    Marke

    PicoKine™

    Analytische Methode

    Quantitative

    Spezifität

    Expression system for standard: NSO
    Immunogen sequence: Q19-G711

    Kreuzreaktivität (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität

    <10pg/mL

    Benötigtes Material

    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

    Immunogen

    Expression system for standard: NSO
    Immunogen sequence: Q19-G711
  • Applikationshinweise

    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

    Kommentare

    Sequence similarities: Belongs to the peptidase S1 family. Plasminogen subfamily.

    Plattentyp

    Pre-coated

    Protokoll

    human MSP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MSP has been precoated onto 96-well plates. Standards(NSO, Q19-G711) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MSP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MSP amount of sample captured in plate.

    Testdurchführung

    Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human MSP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human MSP standard solution and each sample be measured in duplicate.

    Testpräzision

    • Sample 1: n=16, Mean(pg/ml): 1126, Standard deviation: 56.3, CV(%): 5
    • Sample 2: n=16, Mean(pg/ml): 2738, Standard deviation: 170, CV(%): 6.2
    • Sample 3: n=16, Mean(pg/ml): 5273, Standard deviation: 242.6, CV(%): 4.6,
    • Sample 1: n=24, Mean(pg/ml): 1384, Standard deviation: 78.9, CV(%): 5.7
    • Sample 2: n=24, Mean(pg/ml): 2941, Standard deviation: 206, CV(%): 7
    • Sample 3: n=24, Mean(pg/ml): 5527, Standard deviation: 364.8, CV(%): 6.6

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Handhabung

    Avoid multiple freeze-thaw cycles.

    Lagerung

    -20 °C,4 °C

    Informationen zur Lagerung

    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

    Haltbarkeit

    12 months
  • Target Alle MST1 ELISA Kits anzeigen

    MST1 (Macrophage Stimulating 1 (Hepatocyte Growth Factor-Like) (MST1))

    Andere Bezeichnung

    MST1

    Hintergrund

    Background: Macrophage Stimulating 1(SMT1), also known as Hepatocyte growth factor-like protein(HGFL) and MSP, is a protein that in humans is encoded by the MST1 gene. The HGFL gene was identified at the DNF15S2 locus on human chromosome 3(3p21). The RON tyrosine kinase), the receptor for MSP, is expressed on the ciliated epithelia of the mucociliary transport apparatus of the lung. Furthermore, MSP stimulated ciliary motility in these cells by activating RON.

    Synonyms: Hepatocyte growth factor-like protein,Macrophage stimulatory protein,Macrophage-stimulating protein,MSP,Hepatocyte growth factor-like protein alpha chain,Hepatocyte growth factor-like protein beta chain,MST1,D3F15S2, DNF15S2, HGFL,

    Full Gene Name: Hepatocyte growth factor-like protein

    Cellular Localisation: Secreted.

    Gen-ID

    4485

    UniProt

    P26927
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