CCL20 ELISA Kit

Produktnummer ABIN1889300

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Maus CCL20. Es ist 1 Publikation verfügbar.

Kurzübersicht für CCL20 ELISA Kit (ABIN1889300)

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Bindungsspezifität: AA 28-97

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 7.8-500 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Produktdetails CCL20 ELISA Kit

Untere Nachweisgrenze: 7.8 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse MIP-3 alpha/CCL20

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: E.coli
Immunogen sequence: A28-M97

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <1pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: A28-M97

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Tissue Specificity: Prominently expressed in the small intestine, colon and appendix. Also found in thymus, spleen, lymph node and lung. The long form might be dominant in intestinal, and the short form in lymphoid tissues.

Plattentyp: Pre-coated

Protokoll: mouse CCL20 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CCL20 has been precoated onto 96-well plates. Standards(E.coli, A28-M97) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL20 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CCL20 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL mouse CCL20 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse CCL20 standard solution and each sample is measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 59, Standard deviation: 3.66, CV(%): 6.2
• Sample 2: n=16, Mean(pg/ml): 176, Standard deviation: 7.57, CV(%): 4.3
• Sample 3: n=16, Mean(pg/ml): 283, Standard deviation: 15.57, CV(%): 5.5,
• Sample 1: n=24, Mean(pg/ml): 72, Standard deviation: 5.26, CV(%): 7.3
• Sample 2: n=24, Mean(pg/ml): 193, Standard deviation: 11.4, CV(%): 5.9
• Sample 3: n=24, Mean(pg/ml): 317, Standard deviation: 20.3, CV(%): 6.4

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für CCL20 ELISA Kit (ABIN1889300)

Li, Wang, Liu, Meisgen, Grünler, Botusan, Narayanan, Erikci, Li, Blomqvist, Du, Pivarcsi, Sonkoly, Chowdhury, Catrina, Ståhle, Landén: "MicroRNA-132 enhances transition from inflammation to proliferation during wound healing." in: The Journal of clinical investigation, Vol. 125, Issue 8, pp. 3008-26, (2015) (PubMed).

Details zu CCL20

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Andere Bezeichnung: CCL20

Hintergrund:

Protein Function: Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. .

Background: Macrophage Inflammatory Protein 3alpha(MIP3alpha), also called Chemokine, cc motif, ligand 20(CCL20). The MIP-3alpha/CCL20 gene was cloned and sequenced, revealing a four exon, three intron structure, and was localized by FISH analysis to 2q35-q36. MIP3alpha is predominantly expressed in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. MIP3alpha/CCL20 and its receptor CCR6 are markedly up-regulated in psoriasis, and they may play a role in the recruitment of T cells to lesional psoriatic skin. And Alanine MIP-3alpha and Serine MIP-3alpha, the two forms of MIP3alpha, that differ by one amino acid at the predicted signal peptide cleavage site. Both of them were chemically synthesized and tested for biological activity. And both flu antigen plus IL-2-activated CD4(+) and CD8(+) T lymphoblasts and cord blood-derived dendritic cells responded to Ser and Ala MIP-3alpha.

Synonyms: C-C motif chemokine 20,Beta-chemokine exodus-1,CC chemokine LARC,CC chemokine ST38,Liver and activation-regulated chemokine,Macrophage inflammatory protein 3 alpha,MIP-3-alpha,Small-inducible cytokine A20,Ccl20,Larc, Scya20,

Full Gene Name: C-C motif chemokine 20

Cellular Localisation: Secreted.

Gen-ID: 20297

UniProt: O89093

Pathways: Phosphorylierungen bei SARS-CoV-2 Infektion