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SensoLyte® 520 TACE (a-Secretase) Activity Assay Kit

Act, FRET Reaktivität: Human Fluorometric
Produktnummer ABIN1882558
  • Target Alle ADAM17 Kits anzeigen
    ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))
    Reaktivität
    • 2
    • 2
    • 2
    • 1
    Human
    Nachweismethode
    Fluorometric
    Applikation
    Activation (Act), Fluorescence Resonance Energy Transfer Microscopy (FRET)
    Marke
    SensoLyte®
    Produktmerkmale
    SensoLyte® 520 TACE Activity Assay Kit uses a 5-FAM (fluorophore) and QXL™ 520 (quencher) labeled FRET peptide substrate for continuous measurement of enzyme activity. In the intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL™ 520. Upon cleavage of the FRET peptide by the active enzyme, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL™ 520 based FRET peptide has less interference from the autofluorescence of test compounds and cellular components and provides better assay sensitivity. The kit can be used to detect the activity of TACE or screen for TACE inhibitors. The assays are performed in a convenient 96-well format.
    Top Product
    Discover our top product ADAM17 ELISA Kit
  • Kommentare

    FRET-based Assay Kit

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Protect Components A and B from light and from moisture.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Store all kit components at -20 °C. Components C and E can be stored at room temperature for convenience.
  • Kitamura, Srivastava, DiGiovanni, Kiguchi: "Bile acid accelerates erbB2-induced pro-tumorigenic activities in biliary tract cancer." in: Molecular carcinogenesis, Vol. 54, Issue 6, pp. 459-72, (2015) (PubMed).

    Jedicke, Struever, Aggrawal, Welte, Manns, Malek, Zender, Janciauskiene, Wuestefeld: "?-1-antitrypsin inhibits acute liver failure in mice." in: Hepatology (Baltimore, Md.), Vol. 59, Issue 6, pp. 2299-308, (2014) (PubMed).

    Omote, Gohda, Murakoshi, Sasaki, Kazuno, Fujimura, Ishizaka, Sonoda, Tomino: "Role of the TNF pathway in the progression of diabetic nephropathy in KK-A(y) mice." in: American journal of physiology. Renal physiology, Vol. 306, Issue 11, pp. F1335-47, (2014) (PubMed).

    Xu, Padilla, Li, Wells, Kato, Tellez, Belinsky, Kim, Lin: "MUC1 in macrophage: contributions to cigarette smoke-induced lung cancer." in: Cancer research, Vol. 74, Issue 2, pp. 460-70, (2014) (PubMed).

    Rao, Liu, Freedman, Behrens: "Spleen tyrosine kinase (Syk)-dependent calcium signals mediate efficient CpG-induced exocytosis of tumor necrosis factor α (TNFα) in innate immune cells." in: The Journal of biological chemistry, Vol. 288, Issue 18, pp. 12448-58, (2013) (PubMed).

    Menghini, Uccioli, Vainieri, Pecchioli, Casagrande, Stoehr, Cardellini, Porzio, Rizza, Federici: "Expression of tissue inhibitor of metalloprotease 3 is reduced in ischemic but not neuropathic ulcers from patients with type 2 diabetes mellitus." in: Acta diabetologica, Vol. 50, Issue 6, pp. 907-10, (2013) (PubMed).

    Stöhr, Schilbach, Moll, Hettich, Freude, Wunderlich, Ernst, Zemva, Brüning, Krone, Udelhoven, Schubert: "Insulin receptor signaling mediates APP processing and ?-amyloid accumulation without altering survival in a transgenic mouse model of Alzheimer's disease." in: Age (Dordrecht, Netherlands), Vol. 35, Issue 1, pp. 83-101, (2013) (PubMed).

    Fiorentino, Cavalera, Menini, Marchetti, Mavilio, Fabrizi, Conserva, Casagrande, Menghini, Pontrelli, Arisi, DOnofrio, Lauro, Khokha, Accili, Pugliese, Gesualdo, Lauro, Federici: "Loss of TIMP3 underlies diabetic nephropathy via FoxO1/STAT1 interplay." in: EMBO molecular medicine, Vol. 5, Issue 3, pp. 441-55, (2013) (PubMed).

    Foster, Armstrong, Baker, Wong, Wylie, Ramnath, Jenkins, Singh, Steadman, Welsh, Mathieson, Satchell: "Glycosaminoglycan regulation by VEGFA and VEGFC of the glomerular microvascular endothelial cell glycocalyx in vitro." in: The American journal of pathology, Vol. 183, Issue 2, pp. 604-16, (2013) (PubMed).

    Kawasaki, Motoshima, Hanatani, Takaki, Igata, Tsutsumi, Matsumura, Kondo, Senokuchi, Ishii, Kinoshita, Fukuda, Kawashima, Shimoda, Nishikawa, Araki: "Regulation of TNF? converting enzyme activity in visceral adipose tissue of obese mice." in: Biochemical and biophysical research communications, Vol. 430, Issue 4, pp. 1189-94, (2013) (PubMed).

    Lee, Wittki, Bräu, Dreyer, Krätzel, Dindorf, Johnston, Gross, Kremmer, Zeidler, Schlötzer-Schrehardt, Lichtenheld, Saksela, Harrer, Schuler, Federico, Baur: "HIV Nef, paxillin, and Pak1/2 regulate activation and secretion of TACE/ADAM10 proteases." in: Molecular cell, Vol. 49, Issue 4, pp. 668-79, (2013) (PubMed).

    Lockett, Kimani, Ddungu, Wrenger, Tuder, Janciauskiene, Petrache: "??-Antitrypsin modulates lung endothelial cell inflammatory responses to TNF-?." in: American journal of respiratory cell and molecular biology, Vol. 49, Issue 1, pp. 143-50, (2013) (PubMed).

    Wang, Huang, Nayak, Matthews, Warburton, Shi, Sanchez-Esteban: "Strain-induced differentiation of fetal type II epithelial cells is mediated via the integrin ?6?1-ADAM17/tumor necrosis factor-?-converting enzyme (TACE) signaling pathway." in: The Journal of biological chemistry, Vol. 288, Issue 35, pp. 25646-57, (2013) (PubMed).

    Chanthaphavong, Loughran, Lee, Scott, Billiar et al.: "A role for cGMP in inducible nitric-oxide synthase (iNOS)-induced tumor necrosis factor (TNF) α-converting enzyme (TACE/ADAM17) activation, translocation, and TNF receptor 1 (TNFR1) shedding in ..." in: The Journal of biological chemistry, Vol. 287, Issue 43, pp. 35887-98, (2012) (PubMed).

    Jijon, Buret, Hirota, Hollenberg, Beck: "The EGF receptor and HER2 participate in TNF-?-dependent MAPK activation and IL-8 secretion in intestinal epithelial cells." in: Mediators of inflammation, Vol. 2012, pp. 207398, (2012) (PubMed).

    Catalán, Fernández-Castillejo, Anglès, Morelló, Yebras, Solà: "Inhibition of the transcription factor c-Jun by the MAPK family, and not the NF-?B pathway, suggests that peanut extract has anti-inflammatory properties." in: Molecular immunology, Vol. 52, Issue 3-4, pp. 125-32, (2012) (PubMed).

    Costa, Vindeirinho, Cavadas, Ambrósio, Santos: "Contribution of TNF receptor 1 to retinal neural cell death induced by elevated glucose." in: Molecular and cellular neurosciences, Vol. 50, Issue 1, pp. 113-23, (2012) (PubMed).

    Fukaya, Matsui, Tomaru, Kawakami, Sogo, Bohgaki, Atsumi, Koike, Kasahara, Ishizu: "Overexpression of TNF-?-converting enzyme in fibroblasts augments dermal fibrosis after inflammation." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 93, Issue 1, pp. 72-80, (2012) (PubMed).

    Marolda, Ciotti, Matrone, Possenti, Calissano, Cavallaro, Severini: "Substance P activates ADAM9 mRNA expression and induces ?-secretase-mediated amyloid precursor protein cleavage." in: Neuropharmacology, Vol. 62, Issue 5-6, pp. 1954-63, (2012) (PubMed).

    Schmidt, Baum, Lao, Rateitschak, Schmitz, Teichmann, Wiesner, Petersen, Nykjaer, Wolf, Wolkenhauer, Willnow: "Quantitative modelling of amyloidogenic processing and its influence by SORLA in Alzheimer's disease." in: The EMBO journal, Vol. 31, Issue 1, pp. 187-200, (2012) (PubMed).

  • Target
    ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))
    Andere Bezeichnung
    TACE (ADAM17 Produkte)
    Synonyme
    ADAM18 Kit, CD156B Kit, CSVP Kit, NISBD Kit, TACE Kit, CD156b Kit, Tace Kit, adam17 Kit, si:dkey-81b15.2 Kit, adam18 Kit, cd156b Kit, csvp Kit, tace Kit, fc20b01 Kit, wu:fc20b01 Kit, zgc:63886 Kit, ADAM metallopeptidase domain 17 Kit, a disintegrin and metallopeptidase domain 17 Kit, ADAM metallopeptidase domain 17b Kit, ADAM metallopeptidase domain 17 L homeolog Kit, ADAM metallopeptidase domain 17a Kit, ADAM17 Kit, Adam17 Kit, adam17b Kit, adam17.L Kit, adam17a Kit
    Hintergrund
    TACE (tumor necrosis factor-alpha converting enzyme), alpha-secretase, or ADAM17 is a member of the ADAM family of proteases that contain both disintegrin and metalloprotease (catalytic) domains. This enzyme is involved in sperm-egg binding and fusion, muscle cell fusion, neurogenesis, modulation of Notch receptor and ligand processing, and processing of the pro-inflammatory cytokine, TNFalpha. TACE is currently being explored as a target for anti-inflammatory drugs.
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