SensoLyte® 520 Renin Assay Kit

Produktnummer ABIN1882550

Produktdetails

Target: Renin (REN)

Nachweismethode: Fluorometric

Applikation: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Marke: SensoLyte®

Produktmerkmale: The SensoLyte® 520 Renin Assay Kit provides a convenient assay for high throughput screening of renin inhibitors and for continuous assay of renin activity using a 5-FAM/QXL™520 fluorescence resonance energy transfer (FRET) peptide. In the FRET peptide the fluorescence of 5-FAM is quenched by QXL™520. Upon cleavage into two separate fragments by renin, the fluorescence of 5-FAM is recovered, and can be monitored at excitation/emission = 490/520 nm. This assay is about fifty fold more sensitive than an EDANS/DABCYL-based assay and can detect 0.8 ng/mL renin.

Anwendungsinformationen

Kommentare:

FRET-based Assay Kit

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Protect Components A and B from light.

Lagerung: -80 °C

Informationen zur Lagerung: Store all kit components at -20 °C expect for Component C, which should be stored at -80 °C. If used frequently, Component D can be stored at room temperature for convenience.

Referenzen

Schmiedt, Nelson, Brainard, Brown, Vandenplas, Hurley: "Bilateral renal ischemia as a model of acute kidney injury in cats." in: Research in veterinary science, Vol. 93, Issue 2, pp. 950-9, (2012) (PubMed).

Fujita, Ohnishi, Takaoka, Ogasawara, Fukuyama, Nakamuta: "Antihypertensive effects of continuous oral administration of nattokinase and its fragments in spontaneously hypertensive rats." in: Biological & pharmaceutical bulletin, Vol. 34, Issue 11, pp. 1696-701, (2011) (PubMed).

May, Dean, Lowe, Martenyi, Sheehan, Boggs, Monk, Mathes, Mergott, Watson, Stout, Timm, Smith Labell, Gonzales, Nakano, Jhee, Yen, Ereshefsky, Lindstrom, Calligaro, Cocke, Greg Hall, Friedrich, Citron et al.: "Robust central reduction of amyloid-? in humans with an orally available, non-peptidic ?-secretase inhibitor. ..." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 46, pp. 16507-16, (2011) (PubMed).

Sbroggiò, Carnevale, Bertero, Cifelli, De Blasio, Mascio, Hirsch, Bahou, Turco, Silengo, Brancaccio, Lembo, Tarone: "IQGAP1 regulates ERK1/2 and AKT signalling in the heart and sustains functional remodelling upon pressure overload." in: Cardiovascular research, Vol. 91, Issue 3, pp. 456-64, (2011) (PubMed).

Yoshikawa, Aizaki, Kusano, Kishi, Susumu, Iida, Ishiura, Nishimura, Shichiri, Senbonmatsu: "The (pro)renin receptor is cleaved by ADAM19 in the Golgi leading to its secretion into extracellular space." in: Hypertension research : official journal of the Japanese Society of Hypertension, Vol. 34, Issue 5, pp. 599-605, (2011) (PubMed).

Iwai, Kanno, Tomono, Inaba, Senba, Furuno, Mogi, Horiuchi: "Direct renin inhibition improved insulin resistance and adipose tissue dysfunction in type 2 diabetic KK-A(y) mice." in: Journal of hypertension, Vol. 28, Issue 7, pp. 1471-81, (2010) (PubMed).

Feliers, Kasinath: "Mechanism of VEGF expression by high glucose in proximal tubule epithelial cells." in: Molecular and cellular endocrinology, Vol. 314, Issue 1, pp. 136-42, (2009) (PubMed).

Schmiedt, Hurley, Tong, Rakhmanova, Po, Hurley: "Measurement of plasma renin concentration in cats by use of a fluorescence resonance energy transfer peptide substrate of renin." in: American journal of veterinary research, Vol. 70, Issue 11, pp. 1315-22, (2009) (PubMed).

Wei, Ma, Hattori: "Synthesis of dammarane-type triterpene derivatives and their ability to inhibit HIV and HCV proteases." in: Bioorganic & medicinal chemistry, Vol. 17, Issue 8, pp. 3003-10, (2009) (PubMed).

Zivná, H?lková, Matignon, Hodanová, Vyletal, Kalbácová, Baresová, Sikora, Blazková, Zivný, Ivánek, Stránecký, Sovová, Claes, Lerut, Fryns, Hart, Hart, Adams, Pawtowski, Clemessy, Gasc, Gübler et al.: "Dominant renin gene mutations associated with early-onset hyperuricemia, anemia, and chronic kidney failure. ..." in: American journal of human genetics, Vol. 85, Issue 2, pp. 204-13, (2009) (PubMed).

Antigendetails

Target: Renin (REN)

Andere Bezeichnung: Renin (REN Produkte)

Hintergrund: Renin, a highly specific aspartyl protease, cleaves angiotensinogen, to yield angiotensin I, which is further converted into angiotensin II by ACE (Angiotensin Converting Enzyme). Angiotensin II constricts blood vessels leading to increased blood pressure. It also increases the secretion of ADH and aldosterone, and stimulates the hypothalamus to activate the thirst reflex. Since an overactive renin-angiotensin system leads to hypertension, renin is an attractive target for the treatment of this disease.