SensoLyte® 520 MMP-10 Assay Kit

Produktnummer ABIN1882512

Kurzübersicht für SensoLyte® 520 MMP-10 Assay Kit (ABIN1882512)

Target: MMP10 (Matrix Metallopeptidase 10 (Stromelysin 2) (MMP10))

Nachweismethode: Fluorometric

Applikation: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Produktdetails

Marke: SensoLyte®

Produktmerkmale: SensoLyte® 520 MMP-10 Assay Kit uses a 5-FAM (fluorophore) and QXL™520 (quencher) labeled FRET peptide substrates for continuous measurement of the enzyme activities. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL™ 520. Upon the cleavage of the FRET peptide by MMP-10, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL™520 based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity.

Anwendungsinformationen

Kommentare:

FRET-based Assay Kit

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Protect Components A and B from light.

Lagerung: -20 °C

Informationen zur Lagerung: Store all components at -20 °C. Components D and E can be stored at 4 °C for convenience.

Referenzen

De Abrew, Thomas-Virnig, Rasmussen, Bolterstein, Schlosser, Allen-Hoffmann: "TCDD induces dermal accumulation of keratinocyte-derived matrix metalloproteinase-10 in an organotypic model of human skin." in: Toxicology and applied pharmacology, Vol. 276, Issue 3, pp. 171-8, (2014) (PubMed).

Antigendetails

Target: MMP10 (Matrix Metallopeptidase 10 (Stromelysin 2) (MMP10))

Andere Bezeichnung: MMP-10

Hintergrund: MMP-10 (stromelysin 2) cleaves extracellular matrix proteins, but is unable to cleave the triple-helical fibrillar collagens. This enzyme is involved in several pathological conditions, such as cancer, arthritis and wound healing.