HABA Biotin Quantitation Kit

Produktnummer ABIN1882419

Kurzübersicht für HABA Biotin Quantitation Kit (ABIN1882419)

Target: Biotin

Nachweismethode: Colorimetric

Applikation: Enzyme Activity Assay (EAA)

Produktdetails

Konjugat: Biotin

Produktmerkmale: The HABA Biotin Quantitation Kit provides a convenient method for estimating the molar ratio of biotin to protein in biotin-protein conjugates or for quantitating biotin concentration in a solution. The assay employs HABA (4'-hydroxyazobenzene-2-carboxylic acid), a reagent that shows dramatic spectral changes when bound to avidin. Biotin easily displaces HABA from the HABA/Avidin complex, resulting in a decrease of absorption at 500 nm. The kit is best used to determine biotin concentration in the range from 2 to 16 µM. The assay can be performed in a cuvette or microplate format.

Anwendungsinformationen

Kommentare:

Enzymatic Activity Assay kit

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store Component A at -20 °C. Store all other components at 4 °C

Antigendetails

Target: Biotin

Substanzklasse: Chemical