SensoLyte® FDP Alkaline Phosphatase Assay Kit

Produktnummer ABIN1882464

Kurzübersicht für SensoLyte® FDP Alkaline Phosphatase Assay Kit (ABIN1882464)

Target: Fluorescein Diphosphate (FDP)

Nachweismethode: Fluorometric

Applikation: Enzyme Activity Assay (EAA)

Produktdetails

Marke: SensoLyte®

Produktmerkmale: SensoLyte® FDP Alkaline Phosphatase Assay Kit uses highly purified FDP to quantify alkaline phosphatase activity in solutions, in cell extracts, in live cells as well as on solid surfaces (such as PVDF membranes). The kit contains: FDP phosphatase substrate (Ex/Em=485±20/528±20 nm upon dephosphorylation), Calibration standard, Reaction buffer, Stop buffer, An optimized 'mix and read' assay protocol that is compatible with HTS liquid handling instruments

Anwendungsinformationen

Kommentare:

Enzymatic Activity Assay kit

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: Store Component A at -20 °C. Store other kit components at 4 °C.

Referenzen

Zhang, Molenda, Fournelle, Murphy, Sahai: "Effects of pseudowollastonite (CaSiO3) bioceramic on in vitro activity of human mesenchymal stem cells." in: Biomaterials, Vol. 31, Issue 30, pp. 7653-65, (2010) (PubMed).

Arany, Koyota, Sugiyama: "Nerve growth factor promotes differentiation of odontoblast-like cells." in: Journal of cellular biochemistry, Vol. 106, Issue 4, pp. 539-45, (2009) (PubMed).

Antigendetails

Target: Fluorescein Diphosphate (FDP)

Andere Bezeichnung: FDP

Hintergrund: Alkaline phosphatase is widely used in various biological assays (in particular, immunoassays) and ELISA-based diagnostics. Conjugates of calf intestinal alkaline phosphatase are extensively used as secondary detection reagents in ELISAs, immunohistochemical techniques and Northern, Southern and Western blot analyses.