SensoLyte® Rh110 Enterokinase Assay Kit

Produktnummer ABIN1882457

Kurzübersicht für SensoLyte® Rh110 Enterokinase Assay Kit (ABIN1882457)

Target: TMPRSS15 (Transmembrane Protease, serine 15 (TMPRSS15))

Nachweismethode: Fluorometric

Applikation: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Produktdetails

Marke: SensoLyte®

Produktmerkmale: The SensoLyte® Rh110 Enterokinase Assay Kit employs a fluorogenic substrate for the detection of enterokinase activity. Upon protease cleavage, this substrate generates the Rh110 (rhodamine 110) fluorophore with bright green fluorescence that can be detected at excitation/emission=490/520 nm. The longer-wavelength spectra and higher extinction coefficient of the Rh110 provide greater sensitivity and less interference from other reaction components. The assays are performed in a convenient 96-well microplate format.

Anwendungsinformationen

Kommentare:

FRET-based Assay Kit

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Protect Components A and B from light and from moisture.

Lagerung: -80 °C

Informationen zur Lagerung: Store all kit components, except Component C, at -20 °C. Store Component C at -80 °C. Component D can be stored at room temperature for convenience.

Antigendetails

Target: TMPRSS15 (Transmembrane Protease, serine 15 (TMPRSS15))

Andere Bezeichnung: Enterokinase

Hintergrund: Enterokinase or enteropeptidase is a heterodimeric serine protease produced by cells in the duodenal wall. It converts trypsinogen to trypsin, indirectly activating a number of pancreatic digestive enzymes. Enterokinase is a key enzyme for intestinal digestion of proteins. Its deficiency may cause severe protein malabsorption leading to impaired development and growth. Enterokinase also plays an important role in acute experimental and clinical pancreatitis. The high degree of specificity exhibited by enteropeptidase for cleaving affinity or other tags from recombinant proteins makes it an ideal tool for use in biochemical applications.