SensoLyte® Green Elastase Assay Kit

Produktnummer ABIN1882454

Kurzübersicht für SensoLyte® Green Elastase Assay Kit (ABIN1882454)

Target: Elastase

Nachweismethode: Fluorometric

Applikation: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Produktdetails

Marke: SensoLyte®

Produktmerkmale: The SensoLyte® Green Elastase Assay Kit uses natural substrate elastin labeled with the 5-FAM fluorophore and the QXL™ 520 quencher. Proteolytic cleavage of labeled elastin yields brightly green fluorescence, which can be continuously monitored at excitation/emission= 488 nm/520 nm. Increase in fluorescence intensity is directly proportional to elastase activity. This kit does not require any separation steps and can be used to continuously measure the kinetics of elastase activity.

Anwendungsinformationen

Kommentare:

FRET-based Assay Kit

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Protect Component A from light and moisture.

Lagerung: -20 °C

Informationen zur Lagerung: Store all kit components at -20 °C. Component C can be stored at room temperature for convenience.

Antigendetails

Target: Elastase

Hintergrund: Elastases catalyze the hydrolysis of elastin, the chief constituent of elastic fibers that together with collagen determines the mechanical properties of connective tissue. Elastases have many other natural substrates these include proteoglycans, collagens and fibronectin. Implicated in many diseases such as pulmonary emphysema, cystic fibrosis, infections, inflammation and atherosclerosis, elastases are considered important drug targets.