SensoLyte® 520 Cathepsin S Assay Kit

Produktnummer ABIN1882448

Kurzübersicht für SensoLyte® 520 Cathepsin S Assay Kit (ABIN1882448)

Target: Cathepsin S (CTSS)

Nachweismethode: Fluorometric

Applikation: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Produktdetails

Marke: SensoLyte®

Produktmerkmale: The SensoLyte® 520 Cathepsin S Activity Assay Kit uses a 5-FAM/QXL™520 labeled FRET peptide substrate for measurement of enzyme activity. In the intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL™ 520. Upon cleavage of the FRET peptide by the active enzyme, the increase of fluorescence can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, the 5-FAM/QXL™ 520 based FRET peptide shows less interference from the autofluorescence of test compounds and cellular components and provides better assay sensitivity.

Anwendungsinformationen

Kommentare:

FRET-based Assay Kit

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Protect Components A and B from light and moisture.

Lagerung: -80 °C

Informationen zur Lagerung: Store all kit components at -20 °C, except for Component C. Store Component C at -80 °C. Component D can be stored at room temperature for convenience

Referenzen

Chen, Zhang, Zhang, Liang, Wang, Ouyang, Wu, da Fonseca, Weng, Yamamoto, Yamamoto, Natarajan, Badie: "RAGE expression in tumor-associated macrophages promotes angiogenesis in glioma." in: Cancer research, Vol. 74, Issue 24, pp. 7285-97, (2014) (PubMed).

Kim, Jeon, Song, Suh, Kim, Min, Lee: "Synthesis of proline analogues as potent and selective cathepsin S inhibitors." in: Bioorganic & medicinal chemistry letters, Vol. 23, Issue 11, pp. 3140-4, (2013) (PubMed).

Petrey, Flanagan-Steet, Johnson, Fan, De la Rosa, Haskins, Nairn, Moremen, Steet: "Excessive activity of cathepsin K is associated with cartilage defects in a zebrafish model of mucolipidosis II." in: Disease models & mechanisms, Vol. 5, Issue 2, pp. 177-90, (2012) (PubMed).

Antigendetails

Target: Cathepsin S (CTSS)

Andere Bezeichnung: Cathepsin S

Hintergrund: Cathepsins are a class of globular lysosomal proteases playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin S is a cysteine proteinase involved in the pathogenesis of autoimmune diseases, atherosclerosis, cancer, obesity and related diseases.