SensoLyte® 520 Cathepsin E Assay Kit

Produktnummer ABIN1882441

Kurzübersicht für SensoLyte® 520 Cathepsin E Assay Kit (ABIN1882441)

Target: Cathepsin E (CTSE)

Nachweismethode: Fluorometric

Applikation: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Produktdetails

Marke: SensoLyte®

Produktmerkmale: The SensoLyte® 520 Cathepsin E Activity Assay Kit is a homogeneous assay that can be used to detect the enzyme activity in biological samples or in purified enzyme preparations. The unique long wavelength FRET substrate employed in the assay was designed to reduce the cross reactivity of Cathepsin D. A QXL™ 520/HiLyte Fluor™ 488 pair is used for optimal quenching of the intact substrate. When active Cathepsin E cleaves the FRET substrate, it results in an increase of HiLyte Fluor™ 488 fluorescence, monitored at excitation/emission = 490 nm/520 nm. The fluorescent signal from HiLyte Fluor™ 488 is stable at low pH, an optimal pH for cathepsin activity. This assay can detect as low as 2 ng/mL active Cathepsin E.

Anwendungsinformationen

Kommentare:

FRET-based Assay Kit

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Protect Components A and B from light and moisture.

Lagerung: -80 °C

Informationen zur Lagerung: Store all kit components at -20 °C, except Component C. Store Component C at -80 °C. Component D can be stored at room temperature for convenience.

Antigendetails

Target: Cathepsin E (CTSE)

Andere Bezeichnung: Cathepsin E

Hintergrund: Cathepsin E is a non-lysosomal aspartic proteinase of the pepsin superfamily. It plays an important role in the protein degradation, the generation of bioactive proteins, and antigen processing. Recent studies have particularly suggested that Cathepsin E is important in host defense against cancer cells and invading microorganisms.