Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

SensoLyte® 390 Cathepsin D Assay Kit

FRET Fluorometric
Produktnummer ABIN1882438

Kurzübersicht für SensoLyte® 390 Cathepsin D Assay Kit (ABIN1882438)

Target

Alle Cathepsin D (CTSD) Kits anzeigen
Cathepsin D (CTSD)

Nachweismethode

Fluorometric

Applikation

Fluorescence Resonance Energy Transfer Microscopy (FRET)
  • Marke

    SensoLyte®

    Produktmerkmale

    The SensoLyte® 390 Cathepsin D Activity Assay Kit uses a Mca/Dnp FRET peptide for measurement of enzyme activity. In the FRET peptide, the fluorescence of Mca is quenched by Dnp. Upon cleavage into two separate fragments by Cathepsin D, the fluorescence of Mca is recovered, and can be monitored at excitation/emission = 330 nm/390 nm.
  • Kommentare

    FRET-based Assay Kit

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Handhabung

    Protect Components A and B from light and moisture.

    Lagerung

    -80 °C

    Informationen zur Lagerung

    Store all kit components at -20 °C, except for Component C. Store Component C at -80 °C. Component D can be stored at room temperature for convenience
  • Poliakov, Strunnikova, Jiang, Martinez, Parikh, Lakkaraju, Thomas, Brooks, Redmond: "Multiple A2E treatments lead to melanization of rod outer segment-challenged ARPE-19 cells." in: Molecular vision, Vol. 20, pp. 285-300, (2014) (PubMed).

  • Target

    Cathepsin D (CTSD)

    Andere Bezeichnung

    Cathepsin D

    Hintergrund

    Cathepsins are a class of globular lysosomal proteases, playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin D is the lysosomal aspartic proteinase, active in intracellular protein breakdown. It is involved in the pathogenesis of several diseases such as breast cancer and Alzheimer disease.
Sie sind hier:
Chat with us!