SensoLyte® 440 Cathepsin B Assay Kit

Produktnummer ABIN1882437

Kurzübersicht für SensoLyte® 440 Cathepsin B Assay Kit (ABIN1882437)

Target: Cathepsin B (CTSB)

Nachweismethode: Fluorometric

Applikation: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Produktdetails

Marke: SensoLyte®

Produktmerkmale: The SensoLyte® 440 Cathepsin B Assay Kit provides a fluorogenic peptide for measurement of enzyme activity. This peptide releases the AMC (7-amino-4-methylcoumarin) fluorophore upon cathepsin B cleavage and can be detected with excitation at 354 nm and emission at 442 nm.

Anwendungsinformationen

Kommentare:

FRET-based Assay Kit

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Protect Components A and B from light and moisture.

Lagerung: -80 °C

Informationen zur Lagerung: Store all kit components at -20 °C, except for Component C. Store Component C at -80 °C. Component D can be stored at room temperature for convenience

Referenzen

Vidal, Aguiar, Phansalkar, McAlpine, Napolitano, Chen, Araújo, Pauli, Bedran-Russo: "Galloyl moieties enhance the dentin biomodification potential of plant-derived catechins." in: Acta biomaterialia, Vol. 10, Issue 7, pp. 3288-94, (2014) (PubMed).

Antigendetails

Target: Cathepsin B (CTSB)

Andere Bezeichnung: Cathepsin B

Hintergrund: Cathepsins are a class of globular lysosomal proteases playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. The cathepsin family consists of 12 cysteine proteases with broad exo- and endopeptidase activity, of which Cathepsin B is a member. Elevated levels of cathepsin B were detected in metastases and neurological disorders including Alzheimer's disease (AD).