Kurzübersicht für SensoLyte® 520 Cathepsin B Assay Kit (ABIN1882436)
Target
Alle Cathepsin B (CTSB) Kits anzeigenNachweismethode
Applikation
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Marke
- SensoLyte®
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Produktmerkmale
- The SensoLyte® 520 Cathepsin B Activity Assay Kit provides a QXL™ 520/HiLyte FluorTM 488 labeled FRET peptide substrate for measurement of enzyme activity. In the intact FRET peptide, the fluorescence of HiLyte FluorTM 488 is quenched by QXL™ 520. Upon cleavage of the FRET peptide by the active enzyme, the increase of fluorescence can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, the QXL™ 520/HiLyte FluorTM 488 based FRET peptide has less interference from the autofluorescence of test compounds and cellular components and provides better assay sensitivity.
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Kommentare
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FRET-based Assay Kit
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Handhabung
- Protect Components A and B from light and moisture.
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Lagerung
- -80 °C
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Informationen zur Lagerung
- Store all kit components at -20 °C, except for Component C. Store Component C at -80 °C. Component D can be stored at room temperature for convenience
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Development of Purkinje cell degeneration in a knockin mouse model reveals lysosomal involvement in the pathogenesis of SCA6." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 43, pp. 17693-8, (2012) (PubMed).
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Development of Purkinje cell degeneration in a knockin mouse model reveals lysosomal involvement in the pathogenesis of SCA6." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 43, pp. 17693-8, (2012) (PubMed).
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- Cathepsin B (CTSB)
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Andere Bezeichnung
- Cathepsin B
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Hintergrund
- Cathepsins are a class of globular lysosomal proteases, playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin B is a member of the cathepsin family consisting of 12 cysteine proteases with broad exo- and endopeptidase activity. Elevated levels of cathepsin B were detected in metastases and neurological disorders including Alzheimer's disease (AD).
Target
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