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SensoLyte® 520 Cathepsin B Assay Kit

FRET Fluorometric
Produktnummer ABIN1882436

Kurzübersicht für SensoLyte® 520 Cathepsin B Assay Kit (ABIN1882436)

Target

Alle Cathepsin B (CTSB) Kits anzeigen
Cathepsin B (CTSB)

Nachweismethode

Fluorometric

Applikation

Fluorescence Resonance Energy Transfer Microscopy (FRET)
  • Marke

    SensoLyte®

    Produktmerkmale

    The SensoLyte® 520 Cathepsin B Activity Assay Kit provides a QXL™ 520/HiLyte FluorTM 488 labeled FRET peptide substrate for measurement of enzyme activity. In the intact FRET peptide, the fluorescence of HiLyte FluorTM 488 is quenched by QXL™ 520. Upon cleavage of the FRET peptide by the active enzyme, the increase of fluorescence can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, the QXL™ 520/HiLyte FluorTM 488 based FRET peptide has less interference from the autofluorescence of test compounds and cellular components and provides better assay sensitivity.
  • Kommentare

    FRET-based Assay Kit

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Handhabung

    Protect Components A and B from light and moisture.

    Lagerung

    -80 °C

    Informationen zur Lagerung

    Store all kit components at -20 °C, except for Component C. Store Component C at -80 °C. Component D can be stored at room temperature for convenience
  • Unno, Wakamori, Koike, Uchiyama, Ishikawa, Kubota, Yoshida, Sasakawa, Peters, Mizusawa, Watase: "Development of Purkinje cell degeneration in a knockin mouse model reveals lysosomal involvement in the pathogenesis of SCA6." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 43, pp. 17693-8, (2012) (PubMed).

  • Target

    Cathepsin B (CTSB)

    Andere Bezeichnung

    Cathepsin B

    Hintergrund

    Cathepsins are a class of globular lysosomal proteases, playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin B is a member of the cathepsin family consisting of 12 cysteine proteases with broad exo- and endopeptidase activity. Elevated levels of cathepsin B were detected in metastases and neurological disorders including Alzheimer's disease (AD).
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