SensoLyte® Biotin Quantitation Kit

Produktnummer ABIN1882421

Kurzübersicht für SensoLyte® Biotin Quantitation Kit (ABIN1882421)

Target: Biotin

Nachweismethode: Fluorometric

Applikation: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Produktdetails

Konjugat: Biotin

Marke: SensoLyte®

Produktmerkmale: The SensoLyte® Biotin Quantitation Assay Kit provides a convenient assay for estimating the molar ratio of biotin to protein in biotin-protein conjugates or for quantifying biotin concentration in a solution. The assay is based on FRET principle. In the first step, avidin, Reagent A tagged with a quencher dye is briefly incubated with biotin samples/standards. In the second step, fluorescent biotin, Reagent B, is added to the assay mixture. The fluorescent signal is directly proportional to the amount of biotin in the sample and can be detected at Ex/Em=490/520 nm. The kit is best used to determine biotin concentration in the range from 2 to 20 picomoles. The kit also provides biotinylated IgG for use as a positive control and a protease for protein digestion to expose hidden biotin groups.

Anwendungsinformationen

Kommentare:

FRET-based Assay Kit

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Protect Components A and B from light and from moisture.

Lagerung: -20 °C

Informationen zur Lagerung: Store all kit components at -20 °C. Components E can be stored at room temperature for convenience.

Antigendetails

Target: Biotin

Substanzklasse: Chemical