SensoLyte® ONPG b-Galactosidase Assay Kit

Produktnummer ABIN1882416

Kurzübersicht für SensoLyte® ONPG b-Galactosidase Assay Kit (ABIN1882416)

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Nachweismethode: Colorimetric

Applikation: Enzyme Activity Assay (EAA)

Produktdetails

Marke: SensoLyte®

Produktmerkmale: The SensoLyte® ONPG beta-Galactosidase Assay Kit utilizes the widely used chromogenic substrate, o-nitrophenyl beta-D-galactopyranoside (ONPG) to determine the activity of beta-galactosidase in transfected cells or tissues. In the presence of beta-galactosidase, the colorless compound is hydrolyzed into o-nitrophenol, producing a yellow color that can be detected at absorbance 420 nm. The intensity of the color produced is proportional to beta-galactosidase activity.

Anwendungsinformationen

Kommentare:

Enzymatic Activity Assay kit

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Keep component A away from light.

Lagerung: -20 °C

Informationen zur Lagerung: Store all components at -20 °C. Component D can be stored at room temperature for convenience.

Antigendetails

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Andere Bezeichnung: beta-Galactosidase

Substanzklasse: Tag

Hintergrund: Beta-galactosidase, encoded by the lacZ gene in E. coli, is widely used as a reporter enzyme to study gene expression, protein-protein interaction and normalization of transfection efficiency in mammalian cells.