SensoLyte® pNPP Secreted Alkaline Phosphatase Reporter Gene Assay Kit

Produktnummer ABIN1882397

Kurzübersicht für SensoLyte® pNPP Secreted Alkaline Phosphatase Reporter Gene Assay Kit (ABIN1882397)

Target: Alkaline Phosphatase (ALP)

Nachweismethode: Colorimetric

Produktdetails

Marke: SensoLyte®

Produktmerkmale: SensoLyte® pNPP Secreted Alkaline Phosphatase Reporter Gene Assay Kit provides a sensitive colorimetric assay of placental alkaline phosphatase for both secreted and membrane-bound form of enzyme. The kit contains: pNPP chromogenic liquid substrate (absorption maximum = 405 nm upon dephosphorylation), Assay buffer, Stop solution, Placental alkaline phosphatase standard,

Anwendungsinformationen

Kommentare:

Reporter Gene Assay Kit

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Keep Component A away from light.

Lagerung: 4 °C

Informationen zur Lagerung: Store component B and E at -20 °C. Store other kit components at 4 °C.

Referenzen

Cacciatore, Leonard, Chasin: "The isolation of CHO cells with a site conferring a high and reproducible transgene amplification rate." in: Journal of biotechnology, Vol. 164, Issue 2, pp. 346-53, (2013) (PubMed).

Antigendetails

Target: Alkaline Phosphatase (ALP)

Andere Bezeichnung: Alkaline Phosphatase

Hintergrund: The secreted alkaline phosphatase (SEAP) is widely used as reporter gene to analyze gene expression, the activity of promoters and transcriptional factors in cell culture or animals. SEAP can be effectively secreted into culture medium and serum. This unique characteristic of SEAP makes it convenient for kinetic analysis of gene expression over a period of time.