CCL16 ELISA Kit

Produktnummer ABIN1706078

Produktdetails CCL16 ELISA Kit

Target: CCL16 (Chemokine (C-C Motif) Ligand 16 (CCL16))

Bindungsspezifität: AA 24-120

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 7.8-500 pg/mL

Untere Nachweisgrenze: 7.8 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CCL16/HCC-4

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytische Methode: Quantitative

Spezifität: Expression system for standard: E.coli
Immunogen sequence: Q24-Q120

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: Q24-Q120

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Belongs to the intercrine beta (chemokine CC) family.

Tissue Specificity: Mainly expressed in liver, also found in spleen and thymus. Highly expressed in LPS- and IFN-gamma- activated monocytes, weakly in some lymphocytes, including natural killer cells, gamma-delta T-cells, and some T-cell clones.

Plattentyp: Pre-coated

Protokoll: human CCL16 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL16 has been precoated onto 96-well plates. Standards(E.coli, Q24-Q120) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL16 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL16 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL human CCL16 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human CCL16 standard solution and each sample is measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 25, Standard deviation: 0.80, CV(%): 3.2
• Sample 2: n=16, Mean(pg/ml): 76, Standard deviation: 4.26, CV(%): 5.6
• Sample 3: n=16, Mean(pg/ml): 237, Standard deviation: 13.98, CV(%): 5.9,
• Sample 1: n=24, Mean(pg/ml): 26, Standard deviation: 1.51, CV(%): 5.8
• Sample 2: n=24, Mean(pg/ml): 81, Standard deviation: 5.02, CV(%): 6.2
• Sample 3: n=24, Mean(pg/ml): 242, Standard deviation: 16.21, CV(%): 6.7

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu CCL16

Target: CCL16 (Chemokine (C-C Motif) Ligand 16 (CCL16))

Andere Bezeichnung: CCL16 (CCL16 Produkte)

Hintergrund:

Protein Function: Shows chemotactic activity for lymphocytes and monocytes but not neutrophils. Also shows potent myelosuppressive activity, suppresses proliferation of myeloid progenitor cells. Recombinant SCYA16 shows chemotactic activity for monocytes and THP-1 monocytes, but not for resting lymphocytes and neutrophils. Induces a calcium flux in THP-1 cells that were desensitized by prior expression to RANTES.

Background: Chemokine(C-C motif) ligand 16(CCL16) is a small cytokine belonging to the CC chemokine family that is known under several pseudonyms, including Liver-expressed chemokine(LEC) and Monotactin-1(MTN-1). This chemokine is expressed by the liver, thymus, and spleen and is chemoattractive for monocytes and lymphocytes. Cellular expression of CCL16 can be strongly induced in monocytes by IL-10, IFN-gamma and bacterial lipopolysaccharide. Its gene is located on chromosome 17, in humans, among a cluster of other CC chemokines. CCL16 elicits its effects on cells by interacting with cell surface chemokine receptors such as CCR1, CCR2, CCR5 and CCR8.

Synonyms: C-C motif chemokine 16,Chemokine CC-4,HCC-4,Chemokine LEC,IL-10-inducible chemokine,LCC-1,Liver-expressed chemokine,Lymphocyte and monocyte chemoattractant,LMC,Monotactin-1,MTN-1,NCC-4,Small-inducible cytokine A16,CCL16,ILINCK, NCC4, SCYA16,

Full Gene Name: C-C motif chemokine 16

Cellular Localisation: Secreted.

Gen-ID: 6360

UniProt: O15467