Thrombomodulin ELISA Kit

Produktnummer ABIN1706068

Produktdetails Thrombomodulin ELISA Kit

Target: Thrombomodulin (THBD)

Bindungsspezifität: AA 19-515

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5-4000 pg/mL

Untere Nachweisgrenze: 62.5 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Thrombomodulin

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate), Urine

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: A19-S515

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: A19-S515

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Contains 1 C-type lectin domain.

Tissue Specificity: Endothelial cells are unique in synthesizing thrombomodulin.

Plattentyp: Pre-coated

Protokoll: human Thrombomodulin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Thrombomodulin has been precoated onto 96-well plates. Standards(NSO, A19-S515) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Thrombomodulin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Thrombomodulin amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human Thrombomodulin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA, citrate) or urine to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human Thrombomodulin standard solution and each sample is measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 523, Standard deviation: 24.1, CV(%): 4.6
• Sample 2: n=16, Mean(pg/ml): 1074, Standard deviation: 40.1, CV(%): 3.7
• Sample 3: n=16, Mean(pg/ml): 2246, Standard deviation: 92.1, CV(%): 4.1,
• Sample 1: n=24, Mean(pg/ml): 576, Standard deviation: 47.2, CV(%): 8.2
• Sample 2: n=24, Mean(pg/ml): 1187, Standard deviation: 86.7, CV(%): 7.3
• Sample 3: n=24, Mean(pg/ml): 2315, Standard deviation: 180.6, CV(%): 7.8

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für Thrombomodulin ELISA Kit (ABIN1706068)

Kupchak, Kazman, Vingren, Levitt, Lee, Williamson, Armstrong, Deuster: "Blood Hemostatic Changes During an Ultraendurance Road Cycling Event in a Hot Environment." in: Wilderness & environmental medicine, Vol. 28, Issue 3, pp. 197-206, (2018) (PubMed).

Details zu Thrombomodulin

Target: Thrombomodulin (THBD)

Andere Bezeichnung: THBD (THBD Produkte)

Synonyme: AHUS6 ELISA Kit, BDCA3 ELISA Kit, CD141 ELISA Kit, THPH12 ELISA Kit, THRM ELISA Kit, TM ELISA Kit, AI385582 ELISA Kit, THBD ELISA Kit, HAST ELISA Kit, HAST3 ELISA Kit, M-PST ELISA Kit, ST1A3/ST1A4 ELISA Kit, ST1A5 ELISA Kit, STM ELISA Kit, TL-PST ELISA Kit, thrombomodulin ELISA Kit, sulfotransferase family 1A member 3 ELISA Kit, THBD ELISA Kit, Thbd ELISA Kit, SULT1A3 ELISA Kit

Hintergrund:

Protein Function: Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.

Background: Thrombomodulin(TM), CD141 or BDCA-3 is an integral membrane protein expressed on the surface of endothelial cells and serves as a cofactor for thrombin. It reduces blood coagulation by converting thrombin to an anticoagulant enzyme from a procoagulant enzyme. Thrombomodulin is also expressed on human mesothelial cell, monocyte and a dendritic cell subset. In humans, thrombomodulin is encoded by the THBD gene. The protein has a molecular mass of 74 kDa, and consists of a single chain with 5 distinct domains. Thrombomodulin functions as a cofactor in the thrombin-induced activation of protein C in the anticoagulant pathway by forming a 1:1 stoichiometric complex with thrombin. This raises the speed of protein C activation thousandfold. Thrombomodulin-bound thrombin has procoagulant effect at the same time by inhibiting fibrinolysis by cleaving thrombin-activatable fibrinolysis inhibitor(TAFI,aka carboxypeptidase B2) into its active form. Thrombomodulin is a glycoprotein on the surface of endothelial cells that, in addition to binding thrombin, regulates C3b inactivation by factor I. Mutations in the thrombomodulin gene(THBD) have also been reported to be associated with atypical hemolytic-uremic syndrome(aHUS).

Synonyms: Thrombomodulin,TM,Fetomodulin,CD141,THBD,THRM,

Full Gene Name: Thrombomodulin

Cellular Localisation: Membrane, Single-pass type I membrane protein.

Gen-ID: 7056

UniProt: P07204