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PI3 ELISA Kit

This anti-PI3 antibody (ABIN1672903) is a antibody detecting PI3 in ELISA. Suitable for Human.
Produktnummer ABIN1672903

Quick Overview for PI3 ELISA Kit (ABIN1672903)

Target

Alle PI3 ELISA Kits anzeigen
PI3 (Peptidase Inhibitor 3, Skin-Derived (PI3))

Bindungsspezifität

AA 23-117

Reaktivität

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Human

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

31.2-2000 pg/mL

Applikation

ELISA

Proben

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
  • Untere Nachweisgrenze

    31.2 pg/mL

    Verwendungszweck

    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Elafin/PI3

    Marke

    PicoKine™

    Analytische Methode

    Quantitative

    Spezifität

    Expression system for standard: NSO
    Immunogen sequence: A23-Q117

    Kreuzreaktivität (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität

    <10pg/mL

    Benötigtes Material

    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

    Immunogen

    Expression system for standard: NSO
    Immunogen sequence: A23-Q117
  • Applikationshinweise

    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

    Plattentyp

    Pre-coated

    Protokoll

    human Elafin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Elafin has been precoated onto 96-well plates. Standards(NSO, A23-Q117) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Elafin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Elafin amount of sample captured in plate.

    Testdurchführung

    Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human Elafin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum and plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Elafin standard solution and each sample be measured in duplicate.

    Testpräzision

    • Sample 1: n=16, Mean(pg/ml): 225, Standard deviation: 12.6, CV(%): 5.6
    • Sample 2: n=16, Mean(pg/ml): 657, Standard deviation: 32.2, CV(%): 4.9
    • Sample 3: n=16, Mean(pg/ml): 1334, Standard deviation: 57.36, CV(%): 4.3,
    • Sample 1: n=24, Mean(pg/ml): 230, Standard deviation: 17.02, CV(%): 7.4
    • Sample 2: n=24, Mean(pg/ml): 645, Standard deviation: 39.99, CV(%): 6.2
    • Sample 3: n=24, Mean(pg/ml): 1742, Standard deviation: 113.23, CV(%): 6.5

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Handhabung

    Avoid multiple freeze-thaw cycles.

    Lagerung

    -20 °C,4 °C

    Informationen zur Lagerung

    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

    Haltbarkeit

    12 months
  • Target Alle PI3 ELISA Kits anzeigen

    PI3 (Peptidase Inhibitor 3, Skin-Derived (PI3))

    Andere Bezeichnung

    PI3

    Hintergrund

    Protein Function: Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.

    Background: Elafin, also known as peptidase inhibitor 3 or skin-derived antileukoproteinase(SKALP) is a protein that in humans is encoded by the PI3 gene. It is mapped to 20q13.12. This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria. The protein contains a WAP-type four-disulfide core(WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines. This gene is the neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.

    Synonyms: Elafin,Elastase-specific inhibitor,ESI,Peptidase inhibitor 3,PI-3,Protease inhibitor WAP3,Skin-derived antileukoproteinase,SKALP,WAP four-disulfide core domain protein 14,PI3,WAP3, WFDC14,

    Full Gene Name: Elafin

    Cellular Localisation: Secreted.

    Gen-ID

    5266

    UniProt

    P19957
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