IGFBP7 ELISA Kit

Produktnummer ABIN1672887

Dieses Human IGFBP7 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human IGFBP7 zu quantifizieren. Dieses ELISA Kit wurde in 1 Publikation zitiert.

Kurzübersicht für IGFBP7 ELISA Kit (ABIN1672887)

Target: IGFBP7 (Insulin-Like Growth Factor Binding Protein 7 (IGFBP7))

Bindungsspezifität: AA 30-282

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 625-40000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine

Produktdetails IGFBP7 ELISA Kit

Untere Nachweisgrenze: 625 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IGFBP7

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: D30-L282

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <20pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: D30-L282

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Plattentyp: Pre-coated

Protokoll: human IGFBP7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGFBP7 has been precoated onto 96-well plates. Standards(NSO, D30-L282) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IGFBP7 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 40000pg/mL, 20000pg/mL, 10000pg/mL, 5000pg/mL, 2,500pg/mL, 1,250pg/mL, 625pg/mL human IGFBP7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IGFBP7 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(ng/ml): 2.3, Standard deviation: 0.101, CV(%): 4.4
• Sample 2: n=16, Mean(ng/ml): 16.2, Standard deviation: 0.729, CV(%): 4.5
• Sample 3: n=16, Mean(ng/ml): 28.1, Standard deviation: 1.43, CV(%): 5.1,
• Sample 1: n=24, Mean(ng/ml): 2.8, Standard deviation: 0.188, CV(%): 6.7
• Sample 2: n=24, Mean(ng/ml): 15.4, Standard deviation: 1.063, CV(%): 6.9
• Sample 3: n=24, Mean(ng/ml): 29.7, Standard deviation: 2.14, CV(%): 7.2

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für IGFBP7 ELISA Kit (ABIN1672887)

Benassi, Pazzaglia, Novello, Quattrini, Pollino, Magagnoli, Picci, Conti: "Tissue and serum IGFBP7 protein as biomarker in high-grade soft tissue sarcoma." in: American journal of cancer research, Vol. 5, Issue 11, pp. 3446-54, (2016) (PubMed).

Details zu IGFBP7

Target: IGFBP7 (Insulin-Like Growth Factor Binding Protein 7 (IGFBP7))

Andere Bezeichnung: IGFBP7

Hintergrund:

Protein Function: Binds IGF-I and IGF-II with a relatively low affinity. Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion. .

Background: Insulin-like growth factor-binding protein 7 is a protein that in humans is encoded by the IGFBP7 gene. The gene was mapped to chromosome 4q12. The major function of the protein is the regulation of availability of insulin-like growth factors(IGFs) in tissue as well as in modulating IGF binding to its receptors. IGFBP7 binds to IGF with high affinity. It also stimulates cell adhesion. The protein is implicated in some cancers.

Synonyms: Insulin-like growth factor-binding protein 7,IBP-7,IGF-binding protein 7,IGFBP-7,IGFBP-rP1,MAC25 protein,PGI2-stimulating factor,Prostacyclin-stimulating factor,Tumor-derived adhesion factor,TAF,IGFBP7,MAC25, PSF,

Full Gene Name: Insulin-like growth factor-binding protein 7

Cellular Localisation: Secreted.

Gen-ID: 3490

UniProt: Q16270

Pathways: Growth Factor Binding