CHI3L1 ELISA Kit

Produktnummer ABIN1672876

Maus CHI3L1 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Maus CHI3L1.

Kurzübersicht für CHI3L1 ELISA Kit (ABIN1672876)

Target: CHI3L1 (Chitinase 3-Like 1 (Cartilage Glycoprotein-39) (CHI3L1))

Bindungsspezifität: AA 22-381

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5-4000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Produktdetails CHI3L1 ELISA Kit

Untere Nachweisgrenze: 62.5 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Chitinase 3-like 1/YKL-40

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: Y22-A381

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: Y22-A381

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Belongs to the glycosyl hydrolase 18 family.

Tissue Specificity: Detected in lung in pulmonary macrophages and alveolar type 2 cells and in bronchoalveolar lavage (BAL) fluids. Expressed in mammary tumor cells (at protein level). Expressed in lung. Not detected in non-inflammatory colon. .

Plattentyp: Pre-coated

Protokoll: mouse Chitinase 3-like 1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Chitinase 3-like 1 has been precoated onto 96-well plates. Standards(NSO, Y22-A381) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Chitinase 3-like 1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Chitinase 3-like 1 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse Chitinase 3-like 1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse Chitinase 3-like 1 standard solution and each sample is measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 262, Standard deviation: 15.2, CV(%): 5.8
• Sample 2: n=16, Mean(pg/ml): 1559, Standard deviation: 123.16, CV(%): 7.9
• Sample 3: n=16, Mean(pg/ml): 2628, Standard deviation: 134.03, CV(%): 5.1,
• Sample 1: n=24, Mean(pg/ml): 243, Standard deviation: 22.4, CV(%): 9.2
• Sample 2: n=24, Mean(pg/ml): 1524, Standard deviation: 129.54, CV(%): 8.5
• Sample 3: n=24, Mean(pg/ml): 2576, Standard deviation: 190.6, CV(%): 7.4

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu CHI3L1

Target: CHI3L1 (Chitinase 3-Like 1 (Cartilage Glycoprotein-39) (CHI3L1))

Andere Bezeichnung: CHI3L1

Substanzklasse: Viral Protein

Hintergrund:

Protein Function: Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung. .

Background: Chitinase-3-like protein 1, also called CHI3L1 or YKL-40 is a secreted glycoprotein that is approximately 40 kDa in size that in humans is encoded by the CHI3L1 gene. This gene is mapped to 1q32.1. Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling.

Synonyms: Chitinase-3-like protein 1,BRP39 protein,Breast regression protein 39,Cartilage glycoprotein 39,CGP-39,GP-39,Chi3l1,Brp39, Chil1,

Full Gene Name: Chitinase-3-like protein 1

Cellular Localisation: Secreted, extracellular space. Cytoplasm. Endoplasmic reticulum. Detected in bronchoalveolar lavage (BAL) fluids. Localizes mainly to endoplasmic reticulum when overexpressed in cells, with some protein also detected throughout the cytoplasm.

Gen-ID: 12654

UniProt: Q61362