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Granulin ELISA Kit

This anti-Granulin antibody (ABIN1672874) is a antibody detecting Granulin in ELISA. Suitable for Human.
Produktnummer ABIN1672874

Kurzübersicht für Granulin ELISA Kit (ABIN1672874)

Target

Alle Granulin (GRN) ELISA Kits anzeigen
Granulin (GRN)

Bindungsspezifität

AA 18-593

Reaktivität

  • 5
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Human

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

1.56-100 ng/mL

Applikation

ELISA

Proben

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Saliva, Urine, Milk
  • Untere Nachweisgrenze

    1.56 ng/mL

    Verwendungszweck

    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Progranulin

    Marke

    PicoKine™

    Analytische Methode

    Quantitative

    Spezifität

    Expression system for standard: NSO
    Immunogen sequence: T18-L593

    Kreuzreaktivität (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität

    <50pg/mL

    Benötigtes Material

    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

    Immunogen

    Expression system for standard: NSO
    Immunogen sequence: T18-L593
  • Applikationshinweise

    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

    Kommentare

    Sequence similarities: Belongs to the granulin family.

    Tissue Specificity: In myelogenous leukemic cell lines of promonocytic, promyelocytic, and proerythroid lineage, in fibroblasts, and very strongly in epithelial cell lines. Present in inflammatory cells and bone marrow. Highest levels in kidney.

    Plattentyp

    Pre-coated

    Protokoll

    human Progranulin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Progranulin has been precoated onto 96-well plates. Standards(NSO, T18-L593) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Progranulin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Progranulin amount of sample captured in plate.

    Testdurchführung

    Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 1.25 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL human Progranulin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma (heparin, EDTA), saliva, urine or human milk to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human Progranulin standard solution and each sample is measured in duplicate.

    Testpräzision

    • Sample 1: n=16, Mean(ng/ml): 15.4, Standard deviation: 0.986, CV(%): 6.4
    • Sample 2: n=16, Mean(ng/ml): 37.7, Standard deviation: 2, CV(%): 5.3
    • Sample 3: n=16, Mean(ng/ml): 56, Standard deviation: 2.69, CV(%): 4.8,
    • Sample 1: n=24, Mean(ng/ml): 13.6, Standard deviation: 1.02, CV(%): 7.5
    • Sample 2: n=24, Mean(ng/ml): 32.8, Standard deviation: 2.69, CV(%): 8.2
    • Sample 3: n=24, Mean(ng/ml): 61, Standard deviation: 4.76, CV(%): 7.8

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Handhabung

    Avoid multiple freeze-thaw cycles.

    Lagerung

    -20 °C,4 °C

    Informationen zur Lagerung

    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

    Haltbarkeit

    12 months
  • Target Alle Granulin (GRN) ELISA Kits anzeigen

    Granulin (GRN)

    Andere Bezeichnung

    GRN

    Substanzklasse

    Viral Protein

    Hintergrund

    Protein Function: Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling.

    Background: Granulin, also known as Proepithelin(PEPI) is a protein that in humans is encoded by the GRN gene. This gene is mapped to 17q21.31. Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis.

    Synonyms: Granulins,Proepithelin,PEPI,Acrogranin,Glycoprotein of 88 Kda,GP88,Glycoprotein 88,Progranulin,Paragranulin,Granulin-1,Granulin G,Granulin-2,Granulin F,Granulin-3,Granulin B,Granulin-4,Granulin A,Granulin-5,Granulin C,Granulin-6,Granulin D,Granulin-7,Granulin E,GRN,

    Full Gene Name: Granulins

    Cellular Localisation: Secreted.

    Gen-ID

    2896

    UniProt

    P28799
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