RAGE ELISA Kit

Produktnummer ABIN1672873

Ratte RAGE ELISA-Kit Colorimetric-Assay zur Quantifizierung von Ratte RAGE in Cell Culture Supernatant, Serum, Plasma (heparin) und Plasma (EDTA) und wurde in 1 Publikation erwähnt.

Kurzübersicht für RAGE ELISA Kit (ABIN1672873)

Target: RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))

Bindungsspezifität: AA 24-342

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78-5000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Produktdetails RAGE ELISA Kit

Untere Nachweisgrenze: 78 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat RAGE

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: Q24-A342

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: Q24-A342

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

Tissue Specificity: Endothelial cells and cardiomyocytes. .

Plattentyp: Pre-coated

Protokoll: rat RAGE ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for RAGE has been precoated onto 96-well plates. Standards(NSO, Q24-A342) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for RAGE is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat RAGE amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL, 78pg/mL rat RAGE standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each rat RAGE standard solution and each sample is measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 592, Standard deviation: 36.1, CV(%): 6.1
• Sample 2: n=16, Mean(pg/ml): 1590, Standard deviation: 84.27, CV(%): 5.3
• Sample 3: n=16, Mean(pg/ml): 3161, Standard deviation: 180.2, CV(%): 5.7,
• Sample 1: n=24, Mean(pg/ml): 633, Standard deviation: 46.21, CV(%): 7.3
• Sample 2: n=24, Mean(pg/ml): 1386, Standard deviation: 88.71, CV(%): 6.4
• Sample 3: n=24, Mean(pg/ml): 3155, Standard deviation: 227.16, CV(%): 7.2

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für RAGE ELISA Kit (ABIN1672873)

Feng, Zhu, Zhang, Jia, Cheng, Ding, Zhu et al.: "Amelioration of compound 4,4'-diphenylmethane-bis(methyl)carbamate on high mobility group box1-mediated inflammation and oxidant stress responses in human umbilical vein endothelial cells via ..." in: International immunopharmacology, Vol. 15, Issue 2, pp. 206-16, (2013) (PubMed).

Details zu RAGE

Target: RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))

Andere Bezeichnung: AGER

Hintergrund:

Protein Function: Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF- alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. Can also bind oligonucleotides (By similarity). Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. .

Background: RAGE, the Receptor for Advanced Glycation Endproducts, is a 35kD transmembrane receptor of the immunoglobulin super family. It is also known as AGER. AGER gene is mapped to chromosome 6p21.3 by mapping by contiguous cosmids and YAC clones and by fluorescence in situ hybridization. The expression of RAGE is particularly increased in neurons close to deposits of amyloid beta peptide and to neurofibrillary tangles. RAGE has been linked to several chronic diseases, which are thought to result from vascular damage. The pathogenesis is hypothesized to include ligand binding upon which RAGE signals activation of the nuclear factor kappa B(NF-kappaB).

Synonyms: Advanced glycosylation end product-specific receptor,Receptor for advanced glycosylation end products,Ager,Rage,

Full Gene Name: Advanced glycosylation end product-specific receptor

Cellular Localisation: Membrane, Single-pass type I membrane protein.

Gen-ID: 81722

UniProt: Q63495

Pathways: Carbohydrate Homeostasis, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, S100 Proteine