PROCR ELISA Kit

Produktnummer ABIN1672861

Human PROCR ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human PROCR.

Kurzübersicht für PROCR ELISA Kit (ABIN1672861)

Target: PROCR (Protein C Receptor, Endothelial (PROCR))

Bindungsspezifität: AA 18-210

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 312-20000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Produktdetails PROCR ELISA Kit

Untere Nachweisgrenze: 312 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of activated Human EPCR

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: S18-S210

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: S18-S210

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Tissue Specificity: Expressed strongly in the endothelial cells of arteries and veins in heart and lung, less intensely in capillaries in the lung and skin, and not at all in the endothelium of small vessels of the liver and kidney.

Plattentyp: Pre-coated

Protokoll: human EPCR ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for EPCR has been precoated onto 96-well plates. Standards(NSO, S18-S210) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for EPCR is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human EPCR amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human EPCR standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human EPCR standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(ng/ml): 2.21, Standard deviation: 0.095, CV(%): 4.3
• Sample 2: n=16, Mean(ng/ml): 8.45, Standard deviation: 0.313, CV(%): 3.7
• Sample 3: n=16, Mean(ng/ml): 14.4, Standard deviation: 0.691, CV(%): 4.8,
• Sample 1: n=24, Mean(ng/ml): 2.13, Standard deviation: 0.124, CV(%): 5.8
• Sample 2: n=24, Mean(ng/ml): 8.79, Standard deviation: 0.466, CV(%): 5.3
• Sample 3: n=24, Mean(ng/ml): 13.7, Standard deviation: 1.04, CV(%): 7.6

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu PROCR

Target: PROCR (Protein C Receptor, Endothelial (PROCR))

Andere Bezeichnung: PROCR

Hintergrund:

Protein Function: Binds activated protein C. Enhances protein C activation by the thrombin-thrombomodulin complex, plays a role in the protein C pathway controlling blood coagulation.

Background: Endothelial protein C receptor(EPCR) also known as CCCA or PROCR is a protein that in humans is encoded by the PROCR gene. PRPCR gene is mapped to 20q11.2 by radiation hybrid analysis and FISH. The protein encoded by this gene is a receptor for activated protein C, a serine protease activated by and involved in the blood coagulation pathway. The encoded protein is an N-glycosylated type I membrane protein that enhances the activation of protein C. Mutations in this gene have been associated with venous thromboembolism and myocardial infarction, as well as with late fetal loss during pregnancy.

Synonyms: Endothelial protein C receptor,Activated protein C receptor,APC receptor,Endothelial cell protein C receptor,CD201,PROCR,EPCR,

Full Gene Name: Endothelial protein C receptor

Cellular Localisation: Membrane, Single-pass type I membrane protein.

Gen-ID: 10544

UniProt: Q9UNN8