Endothelin 1 ELISA Kit

Produktnummer ABIN1672858

Maus Endothelin 1 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Maus Endothelin 1 in Cell Culture Supernatant, Serum, Plasma (heparin) und Plasma (EDTA) und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für Endothelin 1 ELISA Kit (ABIN1672858)

Target: Endothelin 1 (EDN1)

Bindungsspezifität: AA 53-73

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 3.9-250 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Produktdetails Endothelin 1 ELISA Kit

Untere Nachweisgrenze: 3.9 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Endothelin

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: human peptide
Immunogen sequence: C53-W73

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Homologie: Hamster

Sensitivität: <0.5pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: human peptide
Immunogen sequence: C53-W73

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Tissue Specificity: Highest expression in the adult is in lung. Lower levels found in heart, kidney, brain and intestine. In the embryo, expressed in outer and inner pharyngeal arch surfaces. Also expressed in endothelium of dorsal aorta and arch arteries, and in epithelium of pharyngeal pouches. .

Plattentyp: Pre-coated

Protokoll: mouse Endothelin ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Endothelin has been precoated onto 96-well plates. Standards (mouse peptide,C53-W73) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Endothelin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Endothelin amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 16.5pg/mL, 7.8pg/mL, 3.9pg/mL mouse Endothelin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse Endothelin standard solution and each sample is measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 22.2, Standard deviation: 0.777, CV(%): 3.5
• Sample 2: n=16, Mean(pg/ml): 85.7, Standard deviation: 4.11, CV(%): 4.8
• Sample 3: n=16, Mean(pg/ml): 144, Standard deviation: 8.21, CV(%): 5.7,
• Sample 1: n=24, Mean(pg/ml): 21.4, Standard deviation: 1.46, CV(%): 6.8
• Sample 2: n=24, Mean(pg/ml): 91.5, Standard deviation: 6.68, CV(%): 7.3
• Sample 3: n=24, Mean(pg/ml): 153, Standard deviation: 12.09, CV(%): 7.9

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für Endothelin 1 ELISA Kit (ABIN1672858)

Liu, Zhu, Zhou, Wei, Long, Chen, Ling, Ge, Zhuo: "Endoplasmic reticulum stress promotes amyloid-beta peptides production in RGC-5 cells." in: Cell stress & chaperones, Vol. 19, Issue 6, pp. 827-35, (2014) (PubMed).

Peng, Dai, Ji, Dai: "The separate roles of endothelin receptors participate in remodeling of matrix metalloproteinase and connexin 43 of cardiac fibroblasts in maladaptive response to isoproterenol." in: European journal of pharmacology, Vol. 634, Issue 1-3, pp. 101-6, (2010) (PubMed).

Details zu Endothelin 1

Target: Endothelin 1 (EDN1)

Andere Bezeichnung: EDN1

Hintergrund:

Protein Function: Endothelins are endothelium-derived vasoconstrictor peptides.

Background: The endothelins is a family of structurally and pharmacologically distinct peptides, which has been identified and sequenced in humans. Three isoforms of human endothelin have been identified: endothelin-1, -2, and -3. Endothelin-1 is a potent, 21-amino acid vasoconstrictor peptide produced by vascular endothelial cells. Endothelins are 21-amino acid vasoconstricting peptides produced primarily in the endothelium having a key role in vascular homeostasis. Endothelins are implicated in vascular diseases of several organ systems, including the heart, general circulation and brain. Endothelins are proteins that constrict blood vessels and raise blood pressure. They are normally kept in balance by other mechanisms, but when they are over-expressed, they contribute to high blood pressure(hypertension) and heart disease.

Synonyms: Endothelin-1,ET-1,Preproendothelin-1,PPET1,Edn1,

Full Gene Name: Endothelin-1

Cellular Localisation: Secreted.

Gen-ID: 13614

UniProt: P22387

Pathways: Hormone Transport, Negative Regulation of Hormone Secretion, Regulation of Systemic Arterial Blood Pressure by Hormones, cAMP Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of Cell Size