GDF15 ELISA Kit

Produktnummer ABIN1672794

Dieses Human GDF15 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human GDF15 in Cell Culture Supernatant, Serum, Plasma (heparin) und Plasma (EDTA) zu quantifizieren.

Kurzübersicht für GDF15 ELISA Kit (ABIN1672794)

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Bindungsspezifität: AA 197-308

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2-2000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Produktdetails GDF15 ELISA Kit

Untere Nachweisgrenze: 31.2 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human GDF-15

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: CHO
Immunogen sequence: A197-I308

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: CHO
Immunogen sequence: A197-I308

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Belongs to the TGF-beta family.

Tissue Specificity: Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney.

Plattentyp: Pre-coated

Protokoll: human GDF-15 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for GDF-15 has been precoated onto 96-well plates. Standards(CHO, A197-I308) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GDF-15 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human GDF-15 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human GDF-15 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human GDF-15 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 104, Standard deviation: 2.5, CV(%): 2.4
• Sample 2: n=16, Mean(pg/ml): 735, Standard deviation: 27.93, CV(%): 3.8
• Sample 3: n=16, Mean(pg/ml): 1518, Standard deviation: 68.31, CV(%): 4.5,
• Sample 1: n=24, Mean(pg/ml): 121, Standard deviation: 6.897, CV(%): 5.7
• Sample 2: n=24, Mean(pg/ml): 757, Standard deviation: 46.18, CV(%): 6.1
• Sample 3: n=24, Mean(pg/ml): 1526, Standard deviation: 112.9, CV(%): 7.4

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu GDF15

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Andere Bezeichnung: GDF15

Hintergrund:

Background: GDF-15(Growth differentiation factor 15),also known as TGF-PL, MIC-1, PDF, PLAB, and PTGFB, is a protein belonging to the transforming growth factor beta superfamily that has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. Using FISH, the MIC1 gene is mapped to 19p13.2-p13.1. Its expression in liver can be significantly up-regulated in during injury of organs such as liver, kidney, heart and lung. GDF15 showed increased expression and secretion during erythroblast maturation. GDF15 functions as an antiinflammatory cytokine by directly interfering with chemokine signaling and integrin activation.

Synonyms: Growth/differentiation factor 15,GDF-15,Macrophage inhibitory cytokine 1,MIC-1,NSAID-activated gene 1 protein,NAG-1,NSAID-regulated gene 1 protein,NRG-1,Placental TGF-beta,Placental bone morphogenetic protein,Prostate differentiation factor,GDF15,MIC1, PDF, PLAB, PTGFB,

Full Gene Name: Growth/differentiation factor 15

Cellular Localisation: Secreted.

Gen-ID: 9518

UniProt: Q99988

Pathways: SARS-CoV-2 Protein Interaktom