CXCL11 ELISA Kit

Produktnummer ABIN1672784

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human CXCL11.

Kurzübersicht für CXCL11 ELISA Kit (ABIN1672784)

Target: CXCL11 (Chemokine (C-X-C Motif) Ligand 11 (CXCL11))

Bindungsspezifität: AA 22-94

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5-4000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Produktdetails CXCL11 ELISA Kit

Untere Nachweisgrenze: 62.5 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CXCL11/I-TAC

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: E.coli
Immunogen sequence: F22-F94

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: F22-F94

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Belongs to the intercrine alpha (chemokine CxC) family.

Tissue Specificity: High levels in peripheral blood leukocytes, pancreas and liver astrocytes. Moderate levels in thymus, spleen and lung. Low levels in placenta, prostate and small intestine. Also found in epidermal basal layer keratinocytes in skin disorders.

Plattentyp: Pre-coated

Protokoll: human CXCL11 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL11 has been precoated onto 96-well plates. Standards(E.coli, F22-F94) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL11 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CXCL11 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human CXCL11 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CXCL11 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 512, Standard deviation: 27.1, CV(%): 5.3
• Sample 2: n=16, Mean(pg/ml): 1259, Standard deviation: 65.5, CV(%): 5.2
• Sample 3: n=16, Mean(pg/ml): 2587, Standard deviation: 165.6, CV(%): 6.4,
• Sample 1: n=24, Mean(pg/ml): 524, Standard deviation: 44.54, CV(%): 8.5
• Sample 2: n=24, Mean(pg/ml): 1128, Standard deviation: 88, CV(%): 7.8
• Sample 3: n=24, Mean(pg/ml): 2463, Standard deviation: 199.5, CV(%): 8.1

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu CXCL11

Target: CXCL11 (Chemokine (C-X-C Motif) Ligand 11 (CXCL11))

Andere Bezeichnung: CXCL11

Hintergrund:

Protein Function: Chemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses.

Background: Chemokine(C-X-C motif) ligand 11(CXCL11) is a small cytokine belonging to the CXC chemokine family that is also called Interferon-inducible T-cell alpha chemoattractant(I-TAC) and Interferon-gamma-inducible protein 9(IP-9). By PCR analysis of somatic cell hybrids, the CXCL11 gene was mapped to chromosome 4, and using FISH, it was located to 4q21.2. The CXCL11 was strongly induced by IFNG and beta-interferon, but only weakly induced by alpha-interferon. Induction of CXCL11 required expression of STAT1. What's more, it found that monocyte chemotaxis occurs in response to recombinant CXCL11. RT-PCR analysis detected CXCL11 expression in brain tissue from a patient with AIDS dementia and a patient with multiple sclerosis but not in 2 controls.

Synonyms: C-X-C motif chemokine 11,Beta-R1,H174,Interferon gamma-inducible protein 9,IP-9,Interferon-inducible T-cell alpha chemoattractant,I-TAC,Small-inducible cytokine B11,CXCL11,ITAC, SCYB11, SCYB9B,

Full Gene Name: C-X-C motif chemokine 11

Cellular Localisation: Secreted.

Gen-ID: 6373

UniProt: O14625