CXCL5 ELISA Kit

Produktnummer ABIN1672783

Human CXCL5 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human CXCL5 und wurde in 1 Publikation erwähnt.

Kurzübersicht für CXCL5 ELISA Kit (ABIN1672783)

Target: CXCL5 (Chemokine (C-X-C Motif) Ligand 5 (CXCL5))

Bindungsspezifität: AA 37-114

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2-2000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Produktdetails CXCL5 ELISA Kit

Untere Nachweisgrenze: 31.2 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CXCL5/ENA-78

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: E.coli
Immunogen sequence: A37-N114

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: A37-N114

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Belongs to the intercrine alpha (chemokine CxC) family.

Plattentyp: Pre-coated

Protokoll: human CXCL5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL5 has been precoated onto 96-well plates. Standards(E.coli, A37-N114) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CXCL5 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human CXCL5 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CXCL5 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 214, Standard deviation: 18.19, CV(%): 8.5
• Sample 2: n=16, Mean(pg/ml): 448, Standard deviation: 26.4, CV(%): 5.9
• Sample 3: n=16, Mean(pg/ml): 1094, Standard deviation: 50.32, CV(%): 4.6,
• Sample 1: n=24, Mean(pg/ml): 209, Standard deviation: 18.39, CV(%): 8.8
• Sample 2: n=24, Mean(pg/ml): 442, Standard deviation: 34.9, CV(%): 7.9
• Sample 3: n=24, Mean(pg/ml): 1021, Standard deviation: 72.49, CV(%): 7.1

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für CXCL5 ELISA Kit (ABIN1672783)

Yang, Hou, Shao, Zhang, Qi, E, Wang, Sui, Meng, Wang, Wang, Han, Cao, Huang, Li, Zhang, Wang: "CXCL5 as an autocrine or paracrine cytokine is associated with proliferation and migration of hepatoblastoma HepG2 cells." in: Oncology letters, Vol. 14, Issue 6, pp. 7977-7985, (2017) (PubMed).

Details zu CXCL5

Target: CXCL5 (Chemokine (C-X-C Motif) Ligand 5 (CXCL5))

Andere Bezeichnung: CXCL5

Hintergrund:

Protein Function: Involved in neutrophil activation. In vitro, ENA-78(8- 78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes. .

Background: C-X-C motif chemokine 5 is a protein that in humans encoded by the CXCL5 gene. The protein encoded by this gene, CXCL5 is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78(ENA-78). It is produced following stimulation of cells with the inflammatory cytokines interleukin-1or tumor necrosis factor-alpha. Expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon IFN-gamma. This chemokine stimulates the chemotaxis of neutrophils possessing angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2. The gene for CXCL5 is encoded on four exons and is located on humanchromosome 4 amongst several other CXC chemokine genes. CXCL5 has been implicated in connective tissue remodeling. CXCL5 plays a role in reducing sensitivity to sunburn pain in some subjects, and is a potential target which can be utilized to understand more about pain in other inflammatory conditions like arthritis and cystitis.

Synonyms: C-X-C motif chemokine 5,ENA-78(1-78),Epithelial-derived neutrophil-activating protein 78,Neutrophil-activating peptide ENA-78,Small-inducible cytokine B5,ENA-78(8-78),ENA-78(9-78),CXCL5,ENA78, SCYB5,

Full Gene Name: C-X-C motif chemokine 5

Cellular Localisation: Secreted.

Gen-ID: 6374

UniProt: P42830

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity