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CCL17 ELISA Kit

Dieses Maus CCL17 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Maus CCL17 zu quantifizieren.
Produktnummer ABIN1672770

Kurzübersicht für CCL17 ELISA Kit (ABIN1672770)

Target

Alle CCL17 ELISA Kits anzeigen
CCL17 (Chemokine (C-C Motif) Ligand 17 (CCL17))

Bindungsspezifität

AA 24-93

Reaktivität

  • 6
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Maus

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

31.2-2000 pg/mL

Applikation

ELISA

Proben

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
  • Untere Nachweisgrenze

    31.2 pg/mL

    Verwendungszweck

    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CCL17/TARC

    Marke

    PicoKine™

    Analytische Methode

    Quantitative

    Spezifität

    Expression system for standard: E.coli
    Immunogen sequence: A24-P93

    Kreuzreaktivität (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität

    <10pg/mL

    Benötigtes Material

    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

    Immunogen

    Expression system for standard: E.coli
    Immunogen sequence: A24-P93
  • Applikationshinweise

    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

    Kommentare

    Sequence similarities: Belongs to the intercrine beta (chemokine CC) family.

    Plattentyp

    Pre-coated

    Protokoll

    mouse CCL17 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CCL17 has been precoated onto 96-well plates. Standards(E.coli, A24-P93) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL17 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CCL17 amount of sample captured in plate.

    Testdurchführung

    Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse CCL17 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CCL17 standard solution and each sample be measured in duplicate.

    Testpräzision

    • Sample 1: n=16, Mean(pg/ml): 108, Standard deviation: 5.29, CV(%): 4.9
    • Sample 2: n=16, Mean(pg/ml): 437, Standard deviation: 23.16, CV(%): 5.3
    • Sample 3: n=16, Mean(pg/ml): 1294, Standard deviation: 82.82, CV(%): 6.4,
    • Sample 1: n=24, Mean(pg/ml): 113, Standard deviation: 9.27, CV(%): 8.2
    • Sample 2: n=24, Mean(pg/ml): 472, Standard deviation: 40.59, CV(%): 8.6
    • Sample 3: n=24, Mean(pg/ml): 1302, Standard deviation: 118.48, CV(%): 9.1

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Handhabung

    Avoid multiple freeze-thaw cycles.

    Lagerung

    -20 °C,4 °C

    Informationen zur Lagerung

    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

    Haltbarkeit

    12 months
  • Target Alle CCL17 ELISA Kits anzeigen

    CCL17 (Chemokine (C-C Motif) Ligand 17 (CCL17))

    Andere Bezeichnung

    CCL17

    Hintergrund

    Background: Chemokine(C-C motif) ligand 17(CCL17) is a small cytokine belonging to the CC chemokine family that is also known as thymus and activation regulated chemokine(TARC). CCL17 is expressed constitutively in thymus, but only transiently in phytohemagglutinin-stimulated peripheral blood mononuclear cells. This chemokine specifically binds and induces chemotaxis in T cells and elicits its effects by interacting with the chemokine receptor CCR4. The gene for CCL17 is located on chromosome 16, in humans, along with other chemokines called CCL22 and CX3CL1.

    Synonyms: C-C motif chemokine ,Ccl17 ,

    Full Gene Name: C-C motif chemokine

    Cellular Localisation: Secreted.

    Gen-ID

    20295

    UniProt

    F6R5P4
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