IL-20 ELISA Kit

Produktnummer ABIN1672762

Human IL-20 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human IL-20 in Cell Culture Supernatant, Serum, Plasma (heparin) und Plasma (EDTA) und wurde in 4+ Publikationen erwähnt.

Kurzübersicht für IL-20 ELISA Kit (ABIN1672762)

Target: IL-20 (IL20) (Interleukin 20 (IL20))

Bindungsspezifität: AA 25-176

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5-4000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Produktdetails IL-20 ELISA Kit

Untere Nachweisgrenze: 62.5 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-20

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: E.coli
Immunogen sequence: L25-E176

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: L25-E176

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Belongs to the IL-10 family.

Tissue Specificity: Expressed in most tissues and five major cell types: epithelial cells (primarily skin, buccal mucosa, tongue, nasal mucosa, lung, ureter, breast, prostate, fallopian tube, and adrenal gland), myoepithelial cells (mainly prostate), endothelial cells (mainly in small vessels or capillaries), macrophages, and skeletal muscle. Isoform 2 was detected in the lung tissue only. .

Plattentyp: Pre-coated

Protokoll: human IL-20 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-20 has been precoated onto 96-well plates. Standards(E.coli, L25-E176) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-20 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-20 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human IL-20 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IL-20 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 548, Standard deviation: 35.62, CV(%): 6.5
• Sample 2: n=16, Mean(pg/ml): 1202, Standard deviation: 84.14, CV(%): 7
• Sample 3: n=16, Mean(pg/ml): 2279, Standard deviation: 104.8, CV(%): 4.6,
• Sample 1: n=24, Mean(pg/ml): 623, Standard deviation: 45.5, CV(%): 7.3
• Sample 2: n=24, Mean(pg/ml): 1560, Standard deviation: 117, CV(%): 7.5
• Sample 3: n=24, Mean(pg/ml): 2533, Standard deviation: 142, CV(%): 5.6

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für IL-20 ELISA Kit (ABIN1672762)

Zhou, Jin, Jia, Zhang, Li, Liu, Liu: "Baicalin attenuates brain edema in a rat model of intracerebral hemorrhage." in: Inflammation, Vol. 37, Issue 1, pp. 107-15, (2014) (PubMed).

Liang, Liu, Chen, Sun: "Antitumor activity of the protein and small molecule component fractions from Agrocybe aegerita through enhancement of cytokine production." in: Journal of medicinal food, Vol. 17, Issue 4, pp. 439-46, (2014) (PubMed).

Xia, Du, Wu, Chen, Zhou, Tang, Tang, Liu, Yang, Ruan, Xu, Zuo, Zhang: "E-selectin rs5361 and FCGR2A rs1801274 variants were associated with increased risk of gastric cancer in a Chinese population." in: Molecular carcinogenesis, Vol. 51, Issue 8, pp. 597-607, (2012) (PubMed).

Li, Li, Li, Deng, Tian, Jiang, Wang, Wang: "A rat model for stable chronic obstructive pulmonary disease induced by cigarette smoke inhalation and repetitive bacterial infection." in: Biological & pharmaceutical bulletin, Vol. 35, Issue 10, pp. 1752-60, (2012) (PubMed).

Details zu IL-20

Target: IL-20 (IL20) (Interleukin 20 (IL20))

Andere Bezeichnung: IL20

Hintergrund:

Protein Function: Proinflammatory and angiogenic cytokine that may be involved in epidermal function and psoriasis. Angiogenic and proliferative activities are antagonized by IL10. May act through STAT3. .

Background: Interleukin-20(IL-20) is a protein belonging to the IL-10 family of cytokines. IL-20 is produced by activated keratinocytes and monocytes and transmits an intracellular signal through two distinct cell-surface receptor complexes on keratinocytes and other epithelial cells. By radiation hybrid analysis, the IL20 gene was mapped to chromosome 1q32, where it is tightly linked to the IL10, IL19, and MDA7 genes within a 195-kb region, the IL10 family cytokine cluster. IL-20 regulates proliferation and differentiation of keratinocytes during inflammation, particularly inflammation associated with the skin. In addition, IL-20 also causes cell expansion of multipotential hematopoietic progenitor cells.

Synonyms: Interleukin-20,IL-20,Cytokine Zcyto10,IL20,ZCYTO10,UNQ852/PRO1801,

Full Gene Name: Interleukin-20

Cellular Localisation: Secreted.

Gen-ID: 50604

UniProt: Q9NYY1

Pathways: Protein targeting to Nucleus