SERPINH1 ELISA Kit

Produktnummer ABIN1570373

Produktdetails SERPINH1 ELISA Kit

Target: SERPINH1 (Serpin Family H Member 1 (SERPINH1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 125 pg/mL - 8000 pg/mL

Untere Nachweisgrenze: 125 pg/mL

Applikation: ELISA

Verwendungszweck: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of HSP47 in human serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Proben: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of this index.

Kreuzreaktivität (Details): No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.

Sensitivität: 45.3 pg/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standard (freeze dried)
• Standard Diluent
• Detection Reagent A
• Detection Reagent B
• Assay Diluent A
• Assay Diluent B
• TMB
• Stop Solution
• Wash Buffer (30X)
• Plate sealer for 96 wells
• Instruction manual

Benötigtes Material:

1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Probenmenge: 100 μL

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately.

Testdurchführung:

The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision:

• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
• CV(%) = SD/meanX100
• Intra-assay: CV<10%
• Inter-assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Handhabung: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.

Haltbarkeit: 12 months

Details zu SERPINH1

Target: SERPINH1 (Serpin Family H Member 1 (SERPINH1))

Andere Bezeichnung: Heat Shock Protein 47 (HSP47) (SERPINH1 Produkte)

Synonyme: AsTP3 ELISA Kit, CBP1 ELISA Kit, CBP2 ELISA Kit, HSP47 ELISA Kit, OI10 ELISA Kit, PPROM ELISA Kit, RA-A47 ELISA Kit, SERPINH2 ELISA Kit, gp46 ELISA Kit, SERPINH1 ELISA Kit, DKFZp468B197 ELISA Kit, cb216 ELISA Kit, hsp ELISA Kit, hsp47 ELISA Kit, id:ibd5060 ELISA Kit, wu:fc56b02 ELISA Kit, BERF-1 ELISA Kit, Cbp1 ELISA Kit, Cbp2 ELISA Kit, Hsp47 ELISA Kit, J6 ELISA Kit, Serpinh2 ELISA Kit, colligin ELISA Kit, astp3 ELISA Kit, cbp1 ELISA Kit, cbp2 ELISA Kit, pig14 ELISA Kit, pprom ELISA Kit, ra-a47 ELISA Kit, serpinh2 ELISA Kit, serpin family H member 1 ELISA Kit, serpin peptidase inhibitor, clade H (heat shock protein 47), member 1b ELISA Kit, serpin H1 ELISA Kit, selenoprotein K ELISA Kit, serine (or cysteine) peptidase inhibitor, clade H, member 1 ELISA Kit, serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) L homeolog ELISA Kit, SERPINH1 ELISA Kit, serpinh1b ELISA Kit, LOC100009353 ELISA Kit, Selenok ELISA Kit, Serpinh1 ELISA Kit, serpinh1.L ELISA Kit

Substanzklasse: Viral Protein

Hintergrund: Alternative name: CBP2, AsTP3, CBP1, PIG14, RA-A47, SERPINH1, SERPINH2, Colligen, Gp46, Serpin Peptidase Inhibitor Clade H Member 1, Heat Shock Protein 47, Collagen Binding Protein 2

Gen-ID: 871

UniProt: P50454